ANALYSIS OF EXPRESSION OF MATRIX METALLOPROTEINASE-2 AND METALLOPROTEINASE-9 IN HYPOPHARYNGEAL SQUAMOUS-CELL CARCINOMA BY IN-SITU HYBRIDIZATION

被引：69

作者：

MIYAJIMA, Y

NAKANO, R

MORIMATSU, M

机构：

[1] KURUME UNIV,SCH MED,DEPT PATHOL 2,KURUME,FUKUOKA 830,JAPAN

[2] UNIV OCCUPAT & ENVIRONM HLTH,SCH MED,DEPT PATHOL 2,KITAKYUSHU,FUKUOKA 807,JAPAN

来源：

ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY | 1995年 / 104卷 / 09期

关键词：

HYPOPHARYNGEAL CARCINOMA; MATRIX METALLOPROTEINASE; SQUAMOUS CELL CARCINOMA;

D O I：

10.1177/000348949510400902

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

The gene expression of two type IV collagenases (matrix metalloproteinase [MMP]-2, a 72 kd type TV collagenase, and MMP-9, a 92 kd type TV collagenase) was investigated in carcinomas of the hypopharynx. We examined 27 cases operated on in our hospital by an in situ hybridization technique to detect their messenger RNA signals in cancer cells and surrounding stroma Both signals were detected in all cancer nests and in stromal cells in the same specimens. Clinicopathologic studies showed a significant relationship between MMP-2 expression in the primary cancer and the outcome of treatment. Our present study suggests that hypopharyngeal squamous cell carcinoma producing MMP-2 has a high potential for invasion and metastasis and a poor outcome. The analysis of MMPs will be useful for treatment planning in hypopharyngeal carcinoma and for prognosis.

引用

页码：678 / 684

页数：7

共 20 条

[1] AUTIOHARMAINEN H, 1993, LAB INVEST, V69, P312
[2] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
CHOMCZYNSKI, P
SACCHI, N
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) : 156 - 159
[3] COLLIER IE, 1988, J BIOL CHEM, V263, P6579
[4] Hermanek P, 1987, TNM CLASSIFICATION M, V4, DOI [10.1007/978-3-642-82982-6, DOI 10.1007/978-3-642-82982-6]
[5] HIRANO M, 1982, KURUME MED J, V29, P97
[6] SQUAMOUS-CELL CARCINOMA OF THE HYPOPHARYNX - ANALYSIS OF TREATMENT RESULTS
HO, CM
LAM, KH
WEI, WI
YUEN, PW
LAM, LK
[J]. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 1993, 15 (05): : 405 - 412
[7] NONRADIOACTIVE LABELING OF RNA TRANSCRIPTS INVITRO WITH THE HAPTEN DIGOXIGENIN (DIG) - HYBRIDIZATION AND ELISA-BASED DETECTION
HOLTKE, HJ
KESSLER, C
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (19) : 5843 - 5851
[8] HUHTALA P, 1991, J BIOL CHEM, V266, P16485
[9] ROLE AND REGULATION OF EXPRESSION OF 92-KDA TYPE-IV COLLAGENASE (MMP-9) IN 2 INVASIVE SQUAMOUS-CELL-CARCINOMA CELL-LINES OF THE ORAL CAVITY
JUAREZ, J
CLAYMAN, G
NAKAJIMA, M
TANABE, KK
SAYA, H
NICOLSON, GL
BOYD, D
[J]. INTERNATIONAL JOURNAL OF CANCER, 1993, 55 (01) : 10 - 18
[10] EXPRESSION OF MATRIX METALLOPROTEINASE-2 RELATED TO LYMPH-NODE METASTASIS OF ORAL SQUAMOUS-CELL CARCINOMA - A CLINICOPATHOLOGICAL STUDY
KUSUKAWA, J
SASAGURI, Y
SHIMA, I
KAMEYAMA, T
MORIMATSU, M
[J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1993, 99 (01) : 18 - 23

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