PREIMPLANTATION DEVELOPMENT IN THE STREPTOZOTOCIN-INDUCED DIABETIC MOUSE

被引:10
作者
BEEBE, LFS [1 ]
KAYE, PL [1 ]
机构
[1] UNIV QUEENSLAND,DEPT PHYSIOL & PHARMACOL,ST LUCIA,QLD 4067,AUSTRALIA
基金
英国医学研究理事会;
关键词
Diabetes; Preimplantation embryo;
D O I
10.1071/RD9900407
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Streptozotocin (STZ) was used to develop a diabetic mouse model in which to study the development of the preimplantation embryo. STZ doses of 0, 160, 190, 210 and 240 mg kg-1 were given; 190 mg kg-1 was found to be the most suitable as the standard diabetogenic dose, providing about 60% mice with plasma glucose >20 mM. The STZ-diabetic mice responded to superovulation with 10 i.u. of gonadotrophin in the same manner as control mice, producing similar embryo numbers at 48 h, 72 h and 96 h post-hCG. Furthermore, the proportion of 2-cell embryos collected from STZ-diabetic mice which developed to blastocysts in vitro was similar to that of 2-cell embryos from control mice. The STZ-diabetic mouse model after superovulation thus produced normal early preimplantation embryos whose development can be examined in detail in a diabetic environment. © 1990 CSIRO. All Rights Reserved.
引用
收藏
页码:407 / 412
页数:6
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