HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PROSTACYCLIN

被引：14

作者：

HILL, GT

机构：

[1] Central Analytical Laboratories, The Wellcome Foundation Limited, Dartford,, Kent Kent

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 176卷 / 03期

关键词：

D O I：

10.1016/S0021-9673(00)89459-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the analysis of prostacyclin using a laboratory prepared reversed-phase column packing is described. A relative standard deviation of less than 1% was obtained for ten replicate injections. The system resolve prostacyclin from its hydrolysis product, 6-oxo-prostaglandin F1a and from other prostaglandins present as impurities. These can be estimated to level of approximately 0.5%. The separation of other unrelated prostaglandins by this method is briefly reported. © 1979.

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页码：407 / 412

页数：6

相关论文

共 12 条

[1]

BENEZRA S, 1977, COMMUNICATION

[2] CHEMICAL STABILITY OF PROSTACYCLIN (PGI2) IN AQUEOUS-SOLUTIONS [J].

CHO, MJ ;

ALLEN, MA .

PROSTAGLANDINS, 1978, 15 (06) :943-954

[3]

COREY EJ, 1978, TETRAHEDRON LETT, P1023

[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PROSTAGLANDINS-F2ALPHA, E2, AND D2 FROM INVITRO ENZYME INCUBATIONS [J].

FITZPATRICK, FA .

ANALYTICAL CHEMISTRY, 1976, 48 (03) :499-502

[5] SEPARATION OF PROSTAGLANDINS AND THROMBOXANES BY GAS-CHROMATOGRAPHY WITH GLASS CAPILLARY COLUMNS [J].

FITZPATRICK, FA .

ANALYTICAL CHEMISTRY, 1978, 50 (01) :47-52

[6] SYNTHESIS AND STEREOCHEMISTRY OF PROSTACYCLIN AND SYNTHESIS OF 6-KETOPROSTAGLANDIN-F1ALPHA [J].

JOHNSON, RA ;

LINCOLN, FH ;

THOMPSON, JL ;

NIDY, EG ;

MIZSAK, SA ;

AXEN, U .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1977, 99 (12) :4182-4184

[7] ENZYME ISOLATED FROM ARTERIES TRANSFORMS PROSTAGLANDIN ENDOPEROXIDES TO AN UNSTABLE SUBSTANCE THAT INHIBITS PLATELET-AGGREGATION [J].

MONCADA, S ;

GRYGLEWSKI, R ;

BUNTING, S ;

VANE, JR .

NATURE, 1976, 263 (5579) :663-665

[8] SYNTHESIS OF (4E)-9-DEOXY-6,9ALPHA-EPOXY-DELTA-4-PGF1ALPHA, A PROSTACYCLIN (PGX) ISOMER [J].

NICOLAOU, KC ;

BARNETTE, WE .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1977, (10) :331-332

[9] BUFFERS OF LOW IONIC STRENGTH FOR SPECTROPHOTOMETRIC PK DETERMINATIONS [J].

PERRIN, DD .

AUSTRALIAN JOURNAL OF CHEMISTRY, 1963, 16 (04) :572-&

[10] EFFECTS OF PROSTACYCLIN (PGX) ON CYCLIC-AMP CONCENTRATIONS IN HUMAN PLATELETS [J].

TATESON, JE ;

MONCADA, S ;

VANE, JR .

PROSTAGLANDINS, 1977, 13 (03) :389-397