MORPHOGENIC CELL AND PROTOPLAST CULTURES OF TRITORDEUM

被引：9

作者：

BARCELO, P

LAZZERI, PA

HERNANDEZ, P

MARTIN, A

LORZ, H

机构：

[1] ROTHAMSTED EXPTL STN, HARPENDEN AL5 2JQ, HERTS, ENGLAND

[2] INST AGR SOSTENIBLE, E-14080 CORDOBA, SPAIN

来源：

PLANT SCIENCE | 1993年 / 88卷 / 02期

关键词：

TRITORDEUM; CEREALS; INVITRO REGENERATION; PROTOPLASTS;

D O I：

10.1016/0168-9452(93)90093-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immature embryo- and anther-derived suspension and protoplast cultures were established in hexaploid tritordeum. The morphogenic capability of these cultures was studied under different nitrogen regimes. Both embryo- and anther-derived suspensions showed morphogenic capability. Suspension and protoplast cultures established from immature embryos gave rise to green shoots and green plants, whereas albino plants were regenerated when anther cultures were used as suspension-starting material. Chromosomal stability of suspensions and regenerated plants was analysed, revealing considerable instability in these cultures.

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页码：209 / 218

页数：10

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