INTEGRATION HOST FACTOR STIMULATES THE PHAGE LAMBDA PL PROMOTER

被引：70

作者：

GILADI, H ^{[1
]}

GOTTESMAN, M ^{[1
]}

OPPENHEIM, AB ^{[1
]}

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG,INST CANC RES,NEW YORK,NY 10032

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 213卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0022-2836(05)80124-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli integration host factor (IHF) is a small dimeric protein that binds to a specific DNA consensus sequence and produces DNA bending. Transcription from the bacteriophage lambda pL promoter is stimulated three- to fourfold by IHF both in vivo and in vitro. IHF binds with high-affinity to two tandem sites located just upstream from the pL promoter and enhances the formation of RNA polymerase-promoter closed complexes. The rate of isomerization to open complex is not influenced by IHF. IHF may stimulate recognition of pL by one or more of several mechanisms: (1) by bending DNA; (2) by making protein-protein contacts with RNA polymerase; or (3) by occluding a competing promoter upstream from pL. © 1990 Academic Press Limited.

引用

页码：109 / 121

页数：13

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