INTEGRATION HOST FACTOR STIMULATES THE PHAGE LAMBDA PL PROMOTER

被引：70

作者：

GILADI, H ^{[1
]}

GOTTESMAN, M ^{[1
]}

OPPENHEIM, AB ^{[1
]}

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG,INST CANC RES,NEW YORK,NY 10032

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 213卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0022-2836(05)80124-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli integration host factor (IHF) is a small dimeric protein that binds to a specific DNA consensus sequence and produces DNA bending. Transcription from the bacteriophage lambda pL promoter is stimulated three- to fourfold by IHF both in vivo and in vitro. IHF binds with high-affinity to two tandem sites located just upstream from the pL promoter and enhances the formation of RNA polymerase-promoter closed complexes. The rate of isomerization to open complex is not influenced by IHF. IHF may stimulate recognition of pL by one or more of several mechanisms: (1) by bending DNA; (2) by making protein-protein contacts with RNA polymerase; or (3) by occluding a competing promoter upstream from pL. © 1990 Academic Press Limited.

引用

页码：109 / 121

页数：13

共 51 条

[41] ESCHERICHIA-COLI INTEGRATION HOST FACTOR BENDS THE DNA AT THE ENDS OF IS1 AND IN AN INSERTION HOTSPOT WITH MULTIPLE IHF BINDING-SITES [J].

PRENTKI, P ;

CHANDLER, M ;

GALAS, DJ .

EMBO JOURNAL, 1987, 6 (08) :2479-2487

[42] THE REMARKABLE SPECIFICITY OF A NEW TRANSCRIPTION TERMINATION FACTOR SUGGESTS THAT THE MECHANISMS OF TERMINATION AND ANTITERMINATION ARE SIMILAR [J].

ROBERT, J ;

SLOAN, SB ;

WEISBERG, RA ;

GOTTESMAN, ME ;

ROBLEDO, R ;

HARBRECHT, D .

CELL, 1987, 51 (03) :483-492

[43]

ROBERTSON CA, 1988, J BIOL CHEM, V263, P3554

[44] ON THE ACTION OF THE CYCLIC-AMP CYCLIC-AMP RECEPTOR PROTEIN COMPLEX AT THE ESCHERICHIA-COLI LACTOSE AND GALACTOSE PROMOTER REGIONS [J].

SPASSKY, A ;

BUSBY, S ;

BUC, H .

EMBO JOURNAL, 1984, 3 (01) :43-50

[45] THE INTEGRATION HOST FACTOR OF ESCHERICHIA-COLI BINDS TO BENT DNA AT THE ORIGIN OF REPLICATION OF THE PLASMID-PSC101 [J].

STENZEL, TT ;

PATEL, P ;

BASTIA, D .

CELL, 1987, 49 (05) :709-717

[46] SYNERGY BETWEEN ESCHERICHIA-COLI CAP PROTEIN AND RNA-POLYMERASE IN THE LAC PROMOTER OPEN COMPLEX [J].

STRANEY, DC ;

STRANEY, SB ;

CROTHERS, DM .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 206 (01) :41-57

[47] EMPIRICAL ESTIMATION OF PROTEIN-INDUCED DNA BENDING ANGLES - APPLICATIONS TO LAMBDA-SITE-SPECIFIC RECOMBINATION COMPLEXES [J].

THOMPSON, JF ;

LANDY, A .

NUCLEIC ACIDS RESEARCH, 1988, 16 (20) :9687-9705

[48] INTEGRATION HOST FACTOR (IHF) REPRESSES A CHLAMYDOMONAS CHLOROPLAST PROMOTER IN ESCHERICHIA-COLI [J].

THOMPSON, RJ ;

MOSIG, G .

NUCLEIC ACIDS RESEARCH, 1988, 16 (08) :3313-3326

[49] INTEGRATION HOST FACTOR OF ESCHERICHIA-COLI REGULATES EARLY TRANSCRIPTION AND REPRESSOR TRANSCRIPTION OF BACTERIOPHAGE-MU BY 2 DIFFERENT MECHANISMS [J].

VANRIJN, PA ;

GOOSEN, N ;

VANDEPUTTE, P .

NUCLEIC ACIDS RESEARCH, 1988, 16 (10) :4595-4605

[50]

WU HM, 1984, NATURE, V308, P509, DOI 10.1038/308509a0

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