CONTINUOUS COUNTERACTING CHROMATOGRAPHIC ELECTROPHORESIS

被引：24

作者：

IVORY, CF

GOBIE, WA

机构：

[1] Department of Chemical Engineering, Washington State University, Pullman, Washington

来源：

BIOTECHNOLOGY PROGRESS | 1990年 / 6卷 / 01期

关键词：

D O I：

10.1021/bp00001a004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Counteracting chromatographic electrophoresis (CACE) uses opposing electric and convective fields to focus proteins at the boundary separating distinct granular gel phases. In batch operation, CACE can purify, stack, and concentrate gram quantities of proteins. This technique has been extended to continuous operation by modifying the batch column. Experiments were then used to demonstrate operation of the column. The principles underlying focusing and separation are elucidated by linear chromatography theory. Copyright © 1990 American Institute of Chemical Engineers (AIChE)

引用

页码：21 / 32

页数：12

共 30 条

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