MEASUREMENT OF 5-ANDROSTENE-3-BETA, 17-BETA-DIOL IN PLASMA BY RADIOIMMUNOASSAY

被引:16
作者
MOORE, JW
机构
[1] Department of Clinical Endocrinology, Imperial Cancer Research Fund, London, WC2A 3PX England, Lincoln's Inn Fields
关键词
D O I
10.1016/0022-4731(79)90204-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive and specifie radioimmunoassay for the measurement of 5-androstene-3β 17{amalgamation}-diol (androstenediol) in plasma has been developed using an antiserum against androstenediol-7-carboxymethyloxime conjugated to bovine serum albumin. Plasma samples were extracted in diethylether and chromatographed on Sephadex LH-20 prior to assay. Plasma androstenediol levels were measured in 113 normal women between the ages of 30 and 70 yr. The mean values (±SD) for women in the age ranges 30-50 and 51-70 were 552 ± 223 pg/ml and 401 ± 229 pg/ml respectively. © 1979.
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页码:1329 / 1331
页数:3
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