DETERMINATION OF THE COMPOSITION OF THE FIBRILLAR PART OF GRACILARIA-VERRUCOSA (GRACILARIALES, RHODOPHYTA) CELL-WALL IN ORDER TO PREPARE PROTOPLASTS

被引：15

作者：

BELLANGER, F ^{[1
]}

VERDUS, MC ^{[1
]}

HENOCQ, V ^{[1
]}

CHRISTIAEN, D ^{[1
]}

机构：

[1] UNIV LILLE 1,UFR BIOL,EQUIPE POLYSACCHARIDES PARIETAUX VEGETAUX,BATIMENT SN2,F-59655 VILLENEUVE DASCQ,FRANCE

来源：

HYDROBIOLOGIA | 1990年 / 204卷

关键词：

cell wall; fibrillar component; Gracilaria verrucosa; protoplast; seaweed;

D O I：

10.1007/BF00040281

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

The cell wall of Gracilaria verrucosa is composed of two fractions: a matrix made of agar and a skeleton whose composition is unknown. This fibrillar part was isolated using both physical and chemical techniques. Total hydrolysis followed by gas-liquid chromatography allowed us to establish the sugar composition. Enzymatic degradations were carried out with cellulases, xylanases, agarases and pectinases. Efficiencies of the enzymatic digestions were monitored by both chemical analysis and electron microscopy. Pectinases had no effect. The fibrillar part was composed mainly of a cellulosic network that was unmasked by the xylanase action and degraded after cellulase digestion. The results suggest that a cocktail composed of agarases and cellulases can be used successfully to prepare protoplasts from Gracilaria verrucosa. © 1990 Kluwer Academic Publishers.

引用

页码：527 / 531

页数：5