A NEW CRYSTAL FORM OF THE COMPLEX BETWEEN SERYL-TRANSFER RNA-SYNTHETASE AND TRANSFER RNA(SER) FROM THERMUS-THERMOPHILUS THAT DIFFRACTS TO 2.8-ANGSTROM RESOLUTION

被引：12

作者：

YAREMCHUK, AD

TUKALO, MA

KRIKLIVIY, I

MALCHENKO, N

BIOU, V

BERTHETCOLOMINAS, C

CUSACK, S

机构：

[1] INST MAX VON LAUE PAUL LANGEVIN, EUROPEAN MOLEC BIOL LAB, GRENOBLE OUTSTN, 156X, F-38042 GRENOBLE, FRANCE

[2] ACAD SCI UKRAINE, INST MOLEC BIOL & GENET, KIEV 252627, UKRAINE, USSR

来源：

FEBS LETTERS | 1992年 / 310卷 / 02期

关键词：

AMINOACYL-TRANSFER RNA SYNTHETASE; TRANSFER RNA; PROTEIN RNA COMPLEX; CRYSTALLIZATION; THERMUS-THERMOPHILUS;

D O I：

10.1016/0014-5793(92)81319-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two distinct complexes between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus have been crystallized using ammonium sulphate as a precipitant. Form III crystals grow from solutions containing a 1:2.5 stoichiometry of synthetase dimer to tRNA. They are of monoclinic space group C2 with unit cell dimensions a = 211.6 angstrom, b = 126.8 angstrom, c = 197.1 angstrom, beta = 132.4-degrees and diffract to about 3.5 angstrom. Preliminary crystallographic results show that the crystallographic asymmetric unit contains two synthetase dimers. Form IV crystals grow from solutions containing a 1:1.5 stoichiometry of synthetase dimer to tRNA. They are of orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 124.5 angstrom, b = 128.9 angstrom, c = 121.2 angstrom and diffract to 2.8 angstrom resolution. Preliminary crystallographic results show that these crystals contain only one tRNA molecule bound to a synthetase dimer.

引用

页码：157 / 161

页数：5

共 17 条

[1] AMINOACYL-TERT-RNA SYNTHETASE-INDUCED CLEAVAGE OF TERT-RNA [J].

BERESTEN, S ;

JAHN, M ;

SOLL, D .

NUCLEIC ACIDS RESEARCH, 1992, 20 (07) :1523-1530

[2] SEQUENCE, STRUCTURAL AND EVOLUTIONARY RELATIONSHIPS BETWEEN CLASS-2 AMINOACYL-TRANSFER RNA-SYNTHETASES [J].

CUSACK, S ;

HARTLEIN, M ;

LEBERMAN, R .

NUCLEIC ACIDS RESEARCH, 1991, 19 (13) :3489-3498

[3] A 2ND CLASS OF SYNTHETASE STRUCTURE REVEALED BY X-RAY-ANALYSIS OF ESCHERICHIA-COLI SERYL-TRANSFER RNA-SYNTHETASE AT 2.5-A [J].

CUSACK, S ;

BERTHETCOLOMINAS, C ;

HARTLEIN, M ;

NASSAR, N ;

LEBERMAN, R .

NATURE, 1990, 347 (6290) :249-255

[4] PARTITION OF TRANSFER-RNA SYNTHETASES INTO 2 CLASSES BASED ON MUTUALLY EXCLUSIVE SETS OF SEQUENCE MOTIFS [J].

ERIANI, G ;

DELARUE, M ;

POCH, O ;

GANGLOFF, J ;

MORAS, D .

NATURE, 1990, 347 (6289) :203-206

[5] BENT CRYSTAL, BENT MULTILAYER OPTICS ON A MULTIPOLE WIGGLER LINE FOR AN X-RAY DIFFRACTOMETER WITH AN IMAGING PLATE DETECTOR [J].

FOURME, R ;

DHEZ, P ;

BENOIT, JP ;

KAHN, R ;

DUBUISSON, JM ;

BESSON, P ;

FROUIN, J .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1992, 63 (01) :982-987

[6] EXPERIENCES WITH A NEW TRANSLATION-FUNCTION PROGRAM [J].

FUJINAGA, M ;

READ, RJ .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1987, 20 :517-521

[7] CONVERSION OF AMINOACYLATION SPECIFICITY FROM TRANSFER RNA(TYR) TO TRANSFER RNA(SER) INVITRO [J].

HIMENO, H ;

HASEGAWA, T ;

UEDA, T ;

WATANABE, K ;

SHIMIZU, M .

NUCLEIC ACIDS RESEARCH, 1990, 18 (23) :6815-6819

[8] ESCHERICHIA-COLI SERYL-TRANSFER RNA-SYNTHETASE - THE STRUCTURE OF A CLASS-2 AMINOACYL-TRANSFER RNA-SYNTHETASE [J].

LEBERMAN, R ;

HARTLEIN, M ;

CUSACK, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1089 (03) :287-298

[9]

Leslie AGW, 1992, JOINT CCP4 ESF EACMB

[10] 8 BASE CHANGES ARE SUFFICIENT TO CONVERT A LEUCINE-INSERTING TRANSFER-RNA INTO A SERINE-INSERTING TRANSFER-RNA [J].

NORMANLY, J ;

OLLICK, T ;

ABELSON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5680-5684

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