A NEW CRYSTAL FORM OF THE COMPLEX BETWEEN SERYL-TRANSFER RNA-SYNTHETASE AND TRANSFER RNA(SER) FROM THERMUS-THERMOPHILUS THAT DIFFRACTS TO 2.8-ANGSTROM RESOLUTION

被引：12

作者：

YAREMCHUK, AD

TUKALO, MA

KRIKLIVIY, I

MALCHENKO, N

BIOU, V

BERTHETCOLOMINAS, C

CUSACK, S

机构：

[1] INST MAX VON LAUE PAUL LANGEVIN, EUROPEAN MOLEC BIOL LAB, GRENOBLE OUTSTN, 156X, F-38042 GRENOBLE, FRANCE

[2] ACAD SCI UKRAINE, INST MOLEC BIOL & GENET, KIEV 252627, UKRAINE, USSR

来源：

FEBS LETTERS | 1992年 / 310卷 / 02期

关键词：

AMINOACYL-TRANSFER RNA SYNTHETASE; TRANSFER RNA; PROTEIN RNA COMPLEX; CRYSTALLIZATION; THERMUS-THERMOPHILUS;

D O I：

10.1016/0014-5793(92)81319-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two distinct complexes between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus have been crystallized using ammonium sulphate as a precipitant. Form III crystals grow from solutions containing a 1:2.5 stoichiometry of synthetase dimer to tRNA. They are of monoclinic space group C2 with unit cell dimensions a = 211.6 angstrom, b = 126.8 angstrom, c = 197.1 angstrom, beta = 132.4-degrees and diffract to about 3.5 angstrom. Preliminary crystallographic results show that the crystallographic asymmetric unit contains two synthetase dimers. Form IV crystals grow from solutions containing a 1:1.5 stoichiometry of synthetase dimer to tRNA. They are of orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 124.5 angstrom, b = 128.9 angstrom, c = 121.2 angstrom and diffract to 2.8 angstrom resolution. Preliminary crystallographic results show that these crystals contain only one tRNA molecule bound to a synthetase dimer.

引用

页码：157 / 161

页数：5

共 17 条

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