CONTAMINATION OF AMMONIUM-BASED NUTRIENT SOLUTIONS BY NITRIFYING ORGANISMS AND THE CONVERSION OF AMMONIUM TO NITRATE

被引:42
作者
PADGETT, PE [1 ]
LEONARD, RT [1 ]
机构
[1] UNIV CALIF RIVERSIDE,DEPT BOT & PLANT SCI,RIVERSIDE,CA 92521
关键词
D O I
10.1104/pp.101.1.141
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Conversion of ammonium to nitrate and contamination by nitrifying organisms are often assumed not to be significant in ammonium-based nutrient solutions. To assess this assumption, maize (Zea mays) and pea (Pisum sativum) were grown under greenhouse conditions in aeroponic, hydroponic, and sand-culture systems containing 2 mm ammonium chloride as the sole nitrogen source and evaluated for the activity of contaminating nitrifying organisms. In all three culture systems, root colonization by nitrifying organisms was detected within 5 d, and nitrate was detected in the nutrient solution within 10 d after seedling transfer. In sand culture, solution nitrate concentration reached 0.35 mm by the end of the 17-d experiment. Consistent with the microbial ammonium oxidation sequence, nitrite was detected earlier than nitrate and remained at lower levels throughout the experiment. Nitrate was found in significant quantities in root and shoot tissues from seedlings grown in ammonium-based nutrient solutions in all of the solution culture systems. Maize seedlings grown in an ammonium-based hydroponic system contained nitrate concentrations at 40% of that found in plants grown in nitrate-based solution. Determination of nitrate (or nitrite) levels in the nutrient solution was the weakest indicator of the activity of nitrifying organisms. A bioassay for the presence of nitrifying organisms in combination with tissue analysis for nitrate was a better indicator of microbial conversion of ammonium to nitrate in nutrient solution culture. The results have implications for the use of ammonium-based nutrient solutions to obtain plants suitable for research on induction of nitrate uptake and reduction or for research using solution culture to compare ammonium versus nitrate fertilization.
引用
收藏
页码:141 / 146
页数:6
相关论文
共 27 条
[1]   THE EFFECT OF AN NH4+-ENHANCED NITROGEN-SOURCE ON THE GROWTH AND YIELD OF HYDROPONICALLY GROWN MAIZE (ZEA-MAYS L) [J].
ALEXANDER, KG ;
MILLER, MH ;
BEAUCHAMP, EG .
JOURNAL OF PLANT NUTRITION, 1991, 14 (01) :31-44
[2]   NITRIFICATION IN THE AERATED SUBMERGED FIXED-FILM (ASFF) BIOREACTOR [J].
ALHADDAD, AA ;
ZEIDAN, MO ;
HAMODA, MF .
JOURNAL OF BIOTECHNOLOGY, 1991, 18 (1-2) :115-128
[3]  
ANDREWS M, 1986, PLANT CELL ENVIRON, V9, P511, DOI 10.1111/1365-3040.ep11616228
[4]  
BARBER D A, 1976, Australian Journal of Plant Physiology, V3, P801
[5]   NON-MICROBIAL NITRITE-TO-NITRATE TRANSFORMATION IN SOILS [J].
BARTLETT, RJ .
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1981, 45 (06) :1054-1058
[6]  
Bloom A. J., 1989, Applications of continuous and steady-state methods to root biology., P147
[7]   EFFECTS OF EXPOSURE TO AMMONIUM AND TRANSPLANT SHOCK UPON THE INDUCTION OF NITRATE ABSORPTION [J].
BLOOM, AJ ;
SUKRAPANNA, SS .
PLANT PHYSIOLOGY, 1990, 94 (01) :85-90
[8]  
BROCK TD, 1966, PRINCIPLE MICROBIAL, P81
[9]   ANALYSIS BY DIGESTION AND COLORIMETRIC ASSAY OF TOTAL NITROGEN IN PLANT-TISSUES HIGH IN NITRATE [J].
CATALDO, DA ;
SCHRADER, LE ;
YOUNGS, VL .
CROP SCIENCE, 1974, 14 (06) :854-856
[10]   PLANT-SPECIES RESPONSE TO AMMONIUM-NITRATE CONCENTRATION RATIOS [J].
ERREBHI, M ;
WILCOX, GE .
JOURNAL OF PLANT NUTRITION, 1990, 13 (08) :1017-1029