CYTOLYSINS OF ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPE-9

被引：34

作者：

SMITS, MA ^{[1
]}

BRIAIRE, J ^{[1
]}

JANSEN, R ^{[1
]}

SMITH, HE ^{[1
]}

KAMP, EM ^{[1
]}

GIELKENS, ALJ ^{[1
]}

机构：

[1] CENT VET INST,DEPT BACTERIOL,8200 AB LELYSTAD,NETHERLANDS

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 12期

关键词：

D O I：

10.1128/IAI.59.12.4497-4504.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cytolysin I (ClyI) and cytolysin II (ClyII), which are present in the culture supernatant of Actinobacillus pleuropneumoniae serotype 9, are thought to play an important role in the pathogenesis of pig pleuropneumonia. The purpose of this study was to clone and characterize the genetic determinants of these cytolysins. Cloning was accomplished by the screening of DNA libraries for the presence of cytolytic activity and for the presence of DNA sequences homologous to leukotoxin DNA of Pasteurella haemolytica. Both genetic determinants were found to be members of the RTX cytotoxin family. The ClyII determinant was characterized in more detail. It appeared that ClyII more closely resembled the leukotoxin of P. haemolytica than the alpha-hemolysin of Escherichia coli. The ClyII amino acid sequence was identical to a hemolysin gene sequence of A. pleuropneumoniae serotype 5; this finding indicates that the latter gene also codes for ClyII and not for ClyI, as has previously been suggested. The genetic organization of the ClyII determinant differed from the genetic organization of other RTX determinants. Genes responsible for secretion of ClyII were not contiguous with the toxin gene. Instead, secretion genes were present elsewhere in the genome. These secretion genes, however, belong to the ClyI operon. This indicates that the secretion genes of the ClyI operon are responsible for secretion of ClyI and ClyII.

引用

页码：4497 / 4504

页数：8

共 30 条

[21] ROSENDAL S, 1988, AM J VET RES, V49, P1053
[22] THE CYTOTOXIN OF ACTINOBACILLUS-PLEUROPNEUMONIAE (PLEUROTOXIN) IS DISTINCT FROM THE HEMOLYSIN AND IS ASSOCIATED WITH A 120-KDA POLYPEPTIDE
RYCROFT, AN
WILLIAMS, D
CULLEN, JM
MACDONALD, J
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 : 561 - 568
[23] Sambrook J., 1989, MOL CLONING LAB MANU
[24] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS
SANGER, F
NICKLEN, S
COULSON, AR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) : 5463 - 5467
[25] SEBUNYA TNK, 1983, J AM VET MED ASSOC, V182, P1331
[26] STATHDEE CA, 1987, INFECT IMMUN, V55, P3233
[27] CLONING, NUCLEOTIDE-SEQUENCE, AND CHARACTERIZATION OF GENES ENCODING THE SECRETION FUNCTION OF THE PASTEURELLA-HAEMOLYTICA LEUKOTOXIN DETERMINANT
STRATHDEE, CA
LO, RYC
[J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (02) : 916 - 928
[28] REGULATION OF EXPRESSION OF THE PASTEURELLA-HAEMOLYTICA LEUKOTOXIN DETERMINANT
STRATHDEE, CA
LO, RYC
[J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (11) : 5955 - 5962
[29] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS
TOWBIN, H
STAEHELIN, T
GORDON, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) : 4350 - 4354
[30] VANLEENGOED LAM, 1988, THESIS STATE U UTREC

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