IDENTIFICATION OF THE PHOSPHORYLATION SITES IN ELONGATION FACTOR-II FROM RABBIT RETICULOCYTES

被引：100

作者：

PRICE, NT

REDPATH, NT

SEVERINOV, KV

CAMPBELL, DG

RUSSELL, JM

PROUD, CG

机构：

[1] ACAD SCI USSR,INST PROT RES,PUSHCHINO,USSR

[2] UNIV DUNDEE,DEPT BIOCHEM,MRC,PROT PHOSPHORYLAT GRP,DUNDEE DD1 4HN,SCOTLAND

来源：

FEBS LETTERS | 1991年 / 282卷 / 02期

基金：

英国医学研究理事会;

关键词：

PROTEIN SYNTHESIS; ELONGATION FACTOR-II; PROTEIN PHOSPHORYLATION; PROTEIN KINASE; CALCIUM CALMODULIN; RABBIT RETICULOCYTE;

D O I：

10.1016/0014-5793(91)80489-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sites in eukaryotic elongation factor eEF-2 phosphorylated by the Ca2+/calmodulin-dependent eEF-2 kinase in vitro have been identified. The kinase catalysed the rapid incorporation of one mol of phosphate per mol eEF-2 and the slower incorporation of a second mol. All the phosphorylation sites in eEF-2 are contained in the CNBr fragment corresponding to residues 22-155. Tryptic digestion of phosphorylated eEF-2 yielded 3 phosphopeptides, one being unique to monophosphorylated eEF-2. The phosphorylation sites were identified as threonine residues 56 and 58, the former being more rapidly phosphorylated. Ala-Gly-Glu-Thr-Phe-Thr56-Asp-Thr58-Arg. The same sites are labelled in eEF-2 isolated from reticulocyte lysates.

引用

页码：253 / 258

页数：6

共 30 条

[11]

MACKIE KP, 1989, J BIOL CHEM, V264, P1748

[12]

NAIRN AC, 1985, P NATL ACAD SCI USA, V82, P7939, DOI 10.1073/pnas.82.23.7939

[13]

NAIRN AC, 1987, J BIOL CHEM, V262, P17299

[14]

NAIRN AC, 1987, J BIOL CHEM, V262, P14265

[15]

OLEINIKOV AV, 1989, FEBS LETT, V248, P6384

[16] 3 PHOSPHORYLATION SITES IN ELONGATION FACTOR-II [J].

OVCHINNIKOV, LP ;

MOTUZ, LP ;

NATAPOV, PG ;

AVERBUCH, LJ ;

WETTENHALL, REH ;

SZYSZKA, R ;

KRAMER, G ;

HARDESTY, B .

FEBS LETTERS, 1990, 275 (1-2) :209-212

[17]

PALFREY HC, 1987, J BIOL CHEM, V262, P9785

[18] PHOSPHORYLATION OF PROTEIN-SYNTHESIS INITIATION FACTOR-II - IDENTIFICATION OF THE SITE IN THE ALPHA-SUBUNIT PHOSPHORYLATED IN RETICULOCYTE LYSATES [J].

PRICE, NT ;

PROUD, CG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1054 (01) :83-88

[19] AMINO-ACID SEQUENCES AT 2 SITES ON GLYCOGEN-SYNTHETASE PHOSPHORYLATED BY CYCLIC AMP-DEPENDENT PROTEIN-KINASE AND THEIR DEPHOSPHORYLATION BY PROTEIN PHOSPHATASE-III [J].

PROUD, CG ;

RYLATT, DB ;

YEAMAN, SJ ;

COHEN, P .

FEBS LETTERS, 1977, 80 (02) :435-442

[20] COMPLETE SEQUENCE OF THE CODING REGION OF HUMAN ELONGATION FACTOR-II (EF-2) BY ENZYMATIC AMPLIFICATION OF CDNA FROM HUMAN OVARIAN GRANULOSA-CELLS [J].

RAPP, G ;

KLAUDINY, J ;

HAGENDORFF, G ;

LUCK, MR ;

SCHEIT, KH .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1989, 370 (10) :1071-1075

← 1 2 3 →