GLUCOCORTICOIDS ACCELERATE FETAL MATURATION OF THE EPIDERMAL PERMEABILITY BARRIER IN THE RAT

被引:55
作者
ASZTERBAUM, M
FEINGOLD, KR
MENON, GK
WILLIAMS, ML
机构
[1] DEPT VET AFFAIRS MED CTR, DERMATOL SERV 190, 4150 CLEMENT ST, SAN FRANCISCO, CA 94121 USA
[2] DEPT VET AFFAIRS MED CTR, MED SERV, SAN FRANCISCO, CA 94121 USA
[3] UNIV CALIF SAN FRANCISCO, DEPT DERMATOL, SAN FRANCISCO, CA 94143 USA
[4] UNIV CALIF SAN FRANCISCO, DEPT MED, SAN FRANCISCO, CA 94143 USA
[5] UNIV CALIF SAN FRANCISCO, DEPT PEDIAT, SAN FRANCISCO, CA 94143 USA
关键词
EPIDERMAL LAMELLAR BODY; STRATUM-CORNEUM LIPIDS; TRANSEPIDERMAL WATER LOSS; PERMEABILITY BARRIER;
D O I
10.1172/JCI116509
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The cutaneous permeability barrier to systemic water loss is mediated by hydrophobic lipids forming membrane bilayers within the intercellular domains of the stratum corneum (SC). The barrier emerges during day 20 of gestation in the fetal rat and is correlated with increasing SC thickness and increasing SC lipid content, the appearance of well-formed lamellar bodies in the epidermis, and the presence of lamellar unit structures throughout the SC. Because glucocorticoids accelerate lung lamellar body and surfactant maturation in man and experimental animals, these studies were undertaken to determine whether maternal glucocorticoid treatment accelerates maturation of the epidermal lamellar body secretory system. Maternal rats were injected with betamethasone or saline (control) on days 16-18, and pups were delivered prematurely on day 19. Whereas control pups exhibited immature barriers to transepidermal water loss (8.16+/-0.52 mg/cm2 per h), glucocorticoid-treated pups exhibited competent barriers (0.74+/-0.14 mg/cm2 per h; P < 0.001 ). Glucocorticoid treatment also: (a) accelerated maturation of lamellar body and SC membrane ultrastructure; (b) increased SC total lipid content twofold; and (c) increased cholesterol and polar ceramide content three- to sixfold. Thus, glucocorticoids accelerate the functional, morphological, and lipid biochemical maturation of the permeability barrier in the fetal rat.
引用
收藏
页码:2703 / 2708
页数:6
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