HUMAN COMBINATORIAL ANTIBODY LIBRARIES TO HEPATITIS-B SURFACE-ANTIGEN

被引：153

作者：

ZEBEDEE, SL

BARBAS, CF

HOM, YL

CAOTHIEN, RH

GRAFF, R

DEGRAW, J

PYATI, J

LAPOLLA, R

BURTON, DR

LERNER, RA

THORNTON, GB

机构：

[1] SCRIPPS RES INST, DEPT MOLEC BIOL, LA JOLLA, CA 92037 USA

[2] Scripps Res Inst, DEPT IMMUNOL, LA JOLLA, CA 92037 USA

[3] Scripps Res Inst, DEPT CHEM, LA JOLLA, CA 92037 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 08期

关键词：

D O I：

10.1073/pnas.89.8.3175

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human antibody Fab fragments that bind to hepatitis B surface antigen (HBsAg) were generated by using a recombinant phage surface-display expression system. Characterization of HBsAg-specific Fab fragments isolated from two vaccinated individuals reveals diversity in specificity of antigen binding and in the sequences of the complementarity-determining region. The sequence results show examples of human light-chain promiscuity that result in fine specificity changes and a strong relationship to a human germ-line gene. This application illustrates further that this technique is a powerful tool to isolate distinct human antibodies against immunogenic viral targets.

引用

页码：3175 / 3179

页数：5

共 39 条

[1] SIMPLE, RAPID, AND QUANTITATIVE RELEASE OF PERIPLASMIC PROTEINS BY CHLOROFORM
AMES, GF
PRODY, C
KUSTU, S
[J]. JOURNAL OF BACTERIOLOGY, 1984, 160 (03) : 1181 - 1183
[2] ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE
BARBAS, CF
KANG, AS
LERNER, RA
BENKOVIC, SJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (18) : 7978 - 7982
[3] BARBAS CF, 1991, METHODS COMPANION ME, V2, P119
[4] HORMONE PHAGE - AN ENRICHMENT METHOD FOR VARIANT PROTEINS WITH ALTERED BINDING-PROPERTIES
BASS, S
GREENE, R
WELLS, JA
[J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1990, 8 (04): : 309 - 314
[5] A LARGE ARRAY OF HUMAN MONOCLONAL-ANTIBODIES TO TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS FROM COMBINATORIAL LIBRARIES OF ASYMPTOMATIC SEROPOSITIVE INDIVIDUALS
BURTON, DR
BARBAS, CF
PERSSON, MAA
KOENIG, S
CHANOCK, RM
LERNER, RA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) : 10134 - 10137
[6] INFLUENZA-VIRUS HEMAGGLUTININ-SPECIFIC ANTIBODIES ISOLATED FROM A COMBINATORIAL EXPRESSION LIBRARY ARE CLOSELY RELATED TO THE IMMUNE-RESPONSE OF THE DONOR
CATON, AJ
KOPROWSKI, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) : 6450 - 6454
[7] CATON AJ, 1991, P NATL ACAD SCI USA, V88, P1590
[8] MEMBRANE BIOGENESIS - COTRANSLATIONAL INTEGRATION OF THE BACTERIOPHAGE-F1 COAT PROTEIN INTO AN ESCHERICHIA-COLI MEMBRANE-FRACTION
CHANG, CN
MODEL, P
BLOBEL, G
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (03) : 1251 - 1255
[9] MAKING ANTIBODY FRAGMENTS USING PHAGE DISPLAY LIBRARIES
CLACKSON, T
HOOGENBOOM, HR
GRIFFITHS, AD
WINTER, G
[J]. NATURE, 1991, 352 (6336) : 624 - 628
[10] GENE-III PROTEIN OF FILAMENTOUS PHAGES - EVIDENCE FOR A CARBOXYL-TERMINAL DOMAIN WITH A ROLE IN MORPHOGENESIS
CRISSMAN, JW
SMITH, GP
[J]. VIROLOGY, 1984, 132 (02) : 445 - 455

← 1 2 3 4 →