ZETA-RELATED PROTEIN KINASE-C IN NUCLEI OF NERVE-CELLS

被引：44

作者：

HAGIWARA, M ^{[1
]}

UCHIDA, C ^{[1
]}

USUDA, N ^{[1
]}

NAGATA, T ^{[1
]}

HIDAKA, H ^{[1
]}

机构：

[1] SHINSHU UNIV,DEPT ANAT,MATSUMOTO,NAGANO 390,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 01期

关键词：

D O I：

10.1016/0006-291X(90)91688-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To determine whether or not PKC is present in the nuclei of nerve tissue we made use of biochemical and immunocytochemical techniques. A 219-fold purification of rabbit brain nuclear protein kinase C was achieved by sequential steps of Triton X-100 extraction of isolated nuclei, DEAE-cellulose, Butyl-toyopearl and hydroxylapatite chromatography. The major peak of protein kinase C activity was eluted from the hydroxylapatite column at the KPO4 concentration of 0.3 M. Both Ca2+ and Ptd Ser were required for stimulation of the enzyme. Immunoblot analysis revealed that the kinase fraction was immunoreactive with a polyclonal antibody, PC-ζ, that had been raised against a peptide synthesized according to the deduced sequence of rat ζ protein kinase C. Light-microscopy revealed strong immunoreactivity in the nuclei of Purkinje cells in cerebellum and pyramidal cells in the rat cerebral cortex. These observations suggest that a ζ-related protein kinase C is present in the nuclei of nerve cells. © 1990.

引用

页码：161 / 168

页数：8

共 31 条

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