PREPARATION OF HYDRAZINO-MODIFIED PROTEINS AND THEIR USE FOR THE SYNTHESIS OF TC-99M-PROTEIN CONJUGATES

被引：100

作者：

SCHWARTZ, DA

ABRAMS, MJ

HAUSER, MM

GAUL, FE

LARSEN, SK

RAUH, D

ZUBIETA, JA

机构：

[1] MCNEIL PHARMACEUT RES,SPRING HOUSE,PA 19477

[2] SYRACUSE UNIV,DEPT CHEM,SYRACUSE,NY 13244

来源：

BIOCONJUGATE CHEMISTRY | 1991年 / 2卷 / 05期

关键词：

D O I：

10.1021/bc00011a007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The syntheses and protein linking properties of succinimidyl 4-hydrazinobenzoate hydrochloride (SHBH) and succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH), two new heterobifunctional linkers which lead to hydrazino-modified proteins, are described. SHBH-modified proteins are unstable due to the presence of the phenylhydrazine moiety. This problem was overcome by synthesizing the hydrazinopyridine analogue SHNH, and the conjugates derived from this linker are stable. Tc(V) oxo precursors readily add to hydrazinopyridine-modified proteins to yield the desired Tc-99m-radiolabeled protein. Tc-99m-hydrazinopyridine-polyclonal IgG conjugates are useful agents for the imaging of focal sites of infection.

引用

页码：333 / 336

页数：4

共 27 条

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