INCREASE IN SYNAPTIC VESICLE PROTEINS IN SYNAPSIN-TRANSFECTED NG108-15 CELLS - A SUBCELLULAR FRACTIONATION STUDY

被引:8
作者
FRIED, G [1 ]
HAN, HQ [1 ]
机构
[1] KAROLINSKA INST,DEPT PHYSIOL & PHARMACOL,S-17177 STOCKHOLM,SWEDEN
关键词
SYNAPTIC VESICLES; SYNAPTOPHYSIN; TRANSFERRIN RECEPTOR; ENDOSOMES; SECRETOGRANIN;
D O I
10.1002/syn.890200108
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have examined the subcellular distribution of synapsins and synaptophysin in density gradients from synapsin- and vector-transfected NG108-15 cells, since we recently found that transfection of synapsin IIb, cDNA into neuroblastoma x glioma hybrid cells (NG108-15) resulted in cell lines that had a more neuronal phenotype than controls. The increase in synapsins and synaptophysin in the transfected cells was maximal in the region of the gradient containing small synaptic vesicles. The transferrin receptor, a marker for early endosomes, did not increase in the synapsin-containing fractions in the transfected cells. Secretogranin I, a soluble protein stored in and secreted from large dense cored vesicles, showed a very pronounced increase in the dense regions of gradients from transfected cells. These subcellular fractionation data suggest a possible role for the synapsins in the regulation of synaptic vesicle function. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:44 / 53
页数:10
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