A NEW METHOD FOR QUENCHING CORRECTION LEADS TO REVISIONS OF DATA IN RECEPTOR AUTORADIOGRAPHY

被引：27

作者：

ZILLES, K ^{[1
]}

ZURNIEDEN, K ^{[1
]}

SCHLEICHER, A ^{[1
]}

TRABER, J ^{[1
]}

机构：

[1] TROPONWERKE GMBH & CO KG,DEPT NEUROBIOL,W-5000 COLOGNE 80,GERMANY

来源：

HISTOCHEMISTRY | 1990年 / 94卷 / 06期

关键词：

D O I：

10.1007/BF00271983

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Differential quenching of β-emission affects strongly the analysis of receptor distribution patterns in quantitative receptor autoradiography with tritiated ligands. Different methods for the quenching correction have been described in the past, but some of these are of limited value, if a detailed anatomical parcellation is necessary. Other methods correct exclusively local variations in lipid concentration, which is an important, but only one of several factors causing quenching. A new method for the measurement of quenching (or autoradiographic efficiency) is presented, which permits an anatomically detailed and direct determination of the total quenching without lipid extraction procedures. This method is based on the measurement of autoradiographic efficiency in cryostat sections homogeneously labeled with tritiated formaldehyde by an underlying gelatine section containing this labeled compound. Regional and layer specific measurements of autoradiographic efficiency in cortical and subcortical regions of the human and rat brain are reported. A significant correlation was found between the density of myelin and autoradiographic efficiency but other factors were also shown to influence differential quenching. The use of the here presented correction procedure leads to revisions of the laminar distribution patterns reported for different receptors in human and rat cortical areas. Our results show, that a complete quenching correction is necessary for the mapping of receptor distributions with tritiated ligands. © 1990 Springer-Verlag.

引用

页码：569 / 578

页数：10

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