SYNTHESIS AND CHARACTERIZATION OF A PERTUSSIS TOXIN-BIOTIN CONJUGATE

被引：6

作者：

HEERZE, LD ^{[1
]}

CLARK, CG ^{[1
]}

CHEN, Y ^{[1
]}

SMITH, RH ^{[1
]}

ARMSTRONG, GD ^{[1
]}

机构：

[1] CHEMBIOMED LTD,EDMONTON,ALBERTA,CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0006-291X(91)91737-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We prepared a Pertussis toxin-biotin conjugate and found its biological properties to be similar to those of native Pertussis toxin with respect to the hemagglutination, Chinese hamster ovary cell, and lymphocyte proliferation assays. Direct binding to Chinese hamster ovary and Jurkat cells was observed using fluorescence microscopy. Pertussis toxin-biotin was also found to possess similar glycoconjugate binding specificities as those of 125I -labeled Pertussis toxin. © 1991.

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收藏

页码：1464 / 1469

页数：6

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