CYTOSKELETAL CHANGES AND INDUCTION OF EMBRYOGENESIS IN MICROSPORE AND POLLEN CULTURES OF BRASSICA-NAPUS L

被引：61

作者：

HAUSE, B

HAUSE, G

PECHAN, P

VANLAMMEREN, AAM

机构：

[1] WAGENINGEN UNIV AGR,DEPT PLANT CYTOL & MORPHOL,ARBORETUMLAAN 4,6703 BD WAGENINGEN,NETHERLANDS

[2] MAX PLANCK INST ZUCHTUNGSFORSCH,W-5000 COLOGNE 30,GERMANY

[3] MARTIN LUTHER UNIV HALLE WITTENBERG,INST GENET,O-4020 HALLE,GERMANY

来源：

CELL BIOLOGY INTERNATIONAL | 1993年 / 17卷 / 02期

关键词：

D O I：

10.1006/cbir.1993.1052

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Microspores and pollen of Brassica napus were cultured under conditions leading to embryo formation. Concomitant changes in cytoskeletal configurations were analysed. The microfilamental cytoskeleton exhibited a loss of polarity in embryogenic cells but cytochalasin treatment revealed that microfilaments do not influence embryogenesis. Two embryogenic pathways started from microspores and were either characterized by turned division planes or by division when the nucleus was in the cell centre. In both cases microtubules clearly exhibited new arrangements and likely played a major role in newly induced symmetrical division. In pollen, embryogenic development started in the vegetative cell provided the generative cell was arrested near the pollen wall. The concomitant disappearance of defined microtubular arrays is likely to be responsible for the positioning of the cell. © 1993 Academic Press. All rights reserved.

引用

页码：153 / 168

页数：16

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