THE YERSINIA-PESTIS V-ANTIGEN IS A REGULATORY PROTEIN NECESSARY FOR CA-2+-DEPENDENT GROWTH AND MAXIMAL EXPRESSION OF LOW-CA-2+ RESPONSE VIRULENCE GENES

被引:92
作者
PRICE, SB [1 ]
COWAN, C [1 ]
PERRY, RD [1 ]
STRALEY, SC [1 ]
机构
[1] UNIV KENTUCKY,ALBERT B CHANDLER MED CTR,DEPT MICROBIOL & IMMUNOL,LEXINGTON,KY 40536
关键词
D O I
10.1128/jb.173.8.2649-2657.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The low-Ca2+ response is a multicomponent virulence regulon of the human-pathogenic yersiniae in which 12 known virulence genes are coordinately regulated in response to environmental cues of temperature, Ca2+, and nucleotides such as ATP. Yersinial growth also is regulated, with full growth yield being permitted at 37-degrees-C only if Ca2+ or a nucleotide is present. In this study, we constructed and characterized a mutant Yersinia pestis specifically defective in the gene encoding the V antigen, one of the virulence genes of the low-Ca2+ response. An in-frame internal deletion-insertion mutation was made by removing bases 51 through 645 of lcrV and inserting 61 new bases. The altered lcrV was introduced into the low-Ca2+ response plasmid in Y. pestis by allelic exchange, and the resulting mutant was characterized for its two-dimensional protein profiles, growth, expression of an operon fusion to another low-Ca2+ response virulence operon, and virulence in mice. The mutant had lost its Ca2+ and nucleotide requirement for growth, showed diminished expression of Ca2+- and nucleotide-regulated virulence genes, and was avirulent in mice. The mutation could be complemented with respect to the growth property by supplying native V antigen operon sequences in trans in high copy number (on pBR322). Partial complementation of the growth defect and almost complete complementation of the virulence defect were seen with a lower-copy-number complementing replicon (a pACYC184 derivative). The data are consistent with the interpretation that V antigen is bifunctional, with a role in regulating growth and expression of low-Ca2+ response virulence genes in addition to its putative role as a secreted virulence protein.
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页码:2649 / 2657
页数:9
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