OXIDATIVE DAMAGE TO DNA IN MAMMALIAN CHROMATIN

被引：470

作者：

DIZDAROGLU, M

机构：

[1] Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg

来源：

MUTATION RESEARCH | 1992年 / 275卷 / 3-6期

关键词：

DNA DAMAGE; AGING; HYDROXYL RADICAL; MODIFIED DNA BASES; DNA-PROTEIN CROSS-LINKS; OXYGEN EFFECT;

D O I：

10.1016/0921-8734(92)90036-O

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Efforts have been made to characterize and measure DNA modifications produced in mammalian chromatin in vitro and in vivo by a variety of free radical-producing systems. Methodologies incorporating the technique of gas chromatography/mass spectrometry have been used for this purpose. A number of products from all four DNA bases and several DNA-protein cross-links in isolated chromatin have been identified and quantitated. Product formation has been shown to depend on the free radical-producing system and the presence or absence of oxygen. A similar pattern of DNA modifications has also been observed in chromatin of cultured mammalian cells treated with ionizing radiation or H2O2 and in chromatin of organs of animals treated with carcinogenic metal salts.

引用

页码：331 / 342

页数：12

共 76 条

[61] ESTIMATION OF LIFE TIMES AND DIFFUSION DISTANCES OF RADICALS INVOLVED IN X-RAY-INDUCED DNA STRAND BREAKS OR KILLING OF MAMMALIAN-CELLS [J].