EVIDENCE OF GENETIC-RECOMBINATION IN LEISHMANIA

被引:82
作者
KELLY, JM
LAW, JM
CHAPMAN, CJ
VANEYS, GJJM
EVANS, DA
机构
[1] UNIV LONDON LONDON SCH HYG & TROP MED,DEPT MED PARASITOL,KEPPEL ST,LONDON WC1E 7HT,ENGLAND
[2] ROYAL TROP INST,DEPT TROP HYG,AMSTERDAM,NETHERLANDS
基金
英国医学研究理事会;
关键词
LEISHMANIA; GENETIC EXCHANGE; DNA FINGERPRINTING; PLOIDY;
D O I
10.1016/0166-6851(91)90049-C
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the genus Leishmania there has been no convincing demonstration of genetic exchange, and it has been proposed that reproduction is clonal. However, preliminary characterisation of two strains of Leishmania isolated from wild animals in a zoonotic focus of cutaneous leishmaniasis in the Eastern Province of Saudi Arabia, has suggested that they may represent hybrids of Leishmania major and Leishmania arabica. Evidence presented here strongly supports this hypothesis. Isoenzyme analysis and molecular karyotyping of cloned organisms indicated that the putative hybrids are distinct from other species of Leishmania, and possess characteristics of both L. major and L. arabica. Experiments using highly specific probes demonstrated that kinetoplast minicircle DNA from the putative hybrid contained L. major-specific, but not L. arabica-specific sequences. DNA fingerprinting data obtained using 6 genomic DNA probes were consistent in all cases with a L. major/L. arabica recombinant genotype, and implied both diploidy and allelic segregation. These observations suggest that sexual reproduction may generate genetic diversity within natural Leishmania populations.
引用
收藏
页码:253 / 263
页数:11
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