AUTOCRINE ROLE OF INSULIN-LIKE GROWTH-FACTOR (IGF)-I IN A HUMAN THYROID-CANCER CELL-LINE

被引：41

作者：

ONODA, N ^{[1
]}

OHMURA, E ^{[1
]}

TSUSHIMA, T ^{[1
]}

OHBA, Y ^{[1
]}

EMOTO, N ^{[1
]}

ISOZAKI, O ^{[1
]}

SATO, Y ^{[1
]}

SHIZUME, K ^{[1
]}

DEMURA, H ^{[1
]}

机构：

[1] INST GROWTH SCI,SHINJUKU KU,TOKYO 162,JAPAN

来源：

EUROPEAN JOURNAL OF CANCER | 1992年 / 28A卷 / 11期

关键词：

D O I：

10.1016/0959-8049(92)90033-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

An established cell line (TC-cell, clone 78) derived from human thyroid papillary cancer cells was investigated for production of peptide growth factors. The cells had specific binding sites for insulin-like growth factor-I (IGF-I) and responded to this growth factor with increased proliferation. Culture medium conditioned by TC cells was found to contain insulin-like growth factor (IGF)-I and IGF-binding protein(s). Furthermore, reverse transcription-polymerase chain reaction revealed expression of IGF-I mRNA. When monoclonal antibody to IGF-I receptors (alpha-IR3) was added, the growth of TC cells cultured in serum-free medium was significantly reduced. The growth rate of the cells was restored when the antibody was removed from the medium. These results strongly suggest that TC cells produce IGF-1, which is involved in the regulation of their own growth.

引用

页码：1904 / 1909

页数：6

共 35 条

[11]

Francis T, 1991, Thyroid, V1, P223, DOI 10.1089/thy.1991.1.223

[12] EXPRESSION OF INSULIN-LIKE GROWTH FACTOR-I AND FACTOR-II GENES IN HUMAN SMOOTH-MUSCLE TUMORS [J].