MODE OF ACTION OF (1-]4)-BETA-D-ARABINOXYLAN ARABINOFURANOHYDROLASE (AXH) AND ALPHA-L-ARABINOFURANOSIDASES ON ALKALI-EXTRACTABLE WHEAT-FLOUR ARABINOXYLAN

被引：51

作者：

KORMELINK, FJM ^{[1
]}

GRUPPEN, H ^{[1
]}

VORAGEN, AGJ ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,BIOTECHNION,DEPT FOOD SCI,POB 8129,6700 EV WAGENINGEN,NETHERLANDS

来源：

CARBOHYDRATE RESEARCH | 1993年 / 249卷 / 02期

关键词：

D O I：

10.1016/0008-6215(93)84099-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Arabinoxylan-derived oligosaccharides were treated with (1 --> 4)-beta-D-arabinoxylan arabinofuranohydrolase (AXH) and two types Of alpha-L-arabinofuranosidase, A and B. Analysis of reaction products by high performance anion-exchange chromatography indicated the removal of arabinofuranosyl groups from singly substituted xylopyranosyl residues. In addition, differences in the specificity of these enzymes towards the various differently substituted oligosaccharides were observed. H-1 NMR spectroscopy and methylation analysis of alkali-extractable wheat-flour arabinoxylan treated with AXH confirmed the specificity of AXH towards (1 --> 3)-linked arabinofuranosyl groups on singly substituted xylopyranosyl residues. With these techniques, alpha-L-arabinofuranosidase B was found to cause minor changes in (1 --> 2)- and (1 --> 3)-linked arabinofuranosyl groups on doubly substituted xylopyranosyl residues.

引用

页码：345 / 353

页数：9

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