QUANTITATIVE POLYMERASE CHAIN-REACTION ASSAY FOR AGGRECAN AND LINK PROTEIN GENE-EXPRESSION IN CARTILAGE

被引：28

作者：

RE, P

VALHMU, WB

VOSTREJS, M

HOWELL, DS

FISCHER, SG

RATCLIFFE, A

机构：

[1] COLUMBIA UNIV,DEPT BIOCHEM & MOLEC PHYS,NEW YORK,NY 10027

[2] UNIV MIAMI,CORAL GABLES,FL 33124

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 225卷 / 02期

关键词：

D O I：

10.1006/abio.1995.1169

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure has been developed to quantify the levels of aggrecan and link protein mRNAs in small amounts of various tissues, including cartilage, using the power of PCR to amplify extremely low levels of specific templates. The PCR protocol which was selected allows for a simple assay procedure, with standards in different tubes from the samples. This straightforward procedure is quantitative, inexpensive, and allows for many samples to be analyzed at one time. © 1995 Academic Press, Inc.

引用

页码：356 / 360

页数：5

共 12 条

[1] EXTRACTION AND ISOLATION OF MESSENGER-RNA FROM ADULT ARTICULAR-CARTILAGE [J].