CHARACTERIZATION OF A HUMAN MONOCYTE ANTIGEN, B148.4, REGULATED DURING CELL-DIFFERENTIATION AND ACTIVATION

被引:7
作者
ANEGON, I
BLOTTIERE, H
CUTURI, MC
LENNE, Y
TRINCHIERI, G
FAUST, J
PERUSSIA, B
机构
[1] THOMAS JEFFERSON UNIV,JEFFERSON CANC INST,DEPT MICROBIOL & IMMUNOL,BLSB ROOM 750,PHILADELPHIA,PA 19107
[2] FAC MED NANTES,INSERM,U211,NANTES,FRANCE
[3] CHU NANTES,SERV ANAT PATHOL,NANTES,FRANCE
[4] WISTAR INST ANAT & BIOL,PHILADELPHIA,PA 19104
关键词
MONOCYTES; MONOCLONAL ANTIBODIES; 1-ALPHA; 25-DIHYDROXY-VITAMIN-D(3); DIFFERENTIATION;
D O I
10.1002/jlb.53.4.390
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We analyzed the phenotypic changes associated with monocyte activation and differentiation using a newly developed monoclonal antibody (B148.4). Among peripheral blood leukocytes, the antigen recognized by this antibody is expressed on monocytes and granulocytes at high and low density, respectively. Antigen expression is lost during in vitro differentiation of monocytes and is absent on tissue macrophages, indicating that expression of this antigen is related to monocyte differentiation. Only 1alpha,25-dihydroxyvitamin D3 and phorbol diesters, of several inducers tested, up-regulate B148.4 antigen expression on cells (monocytes and certain myeloid cell lines) that constitutively bear the antigen, without, however, allowing its maintenance during monocytic differentiation or inducing it on negative cells. By immunoprecipitation from B148.4+ U937 cells, the antigen is a complex of a major 116-kd and two minor 38- and 46-kd molecules. Analysis of eight different tissues reveals that the antigen is shared with endothelial cells. Biochemical characteristics, cellular distribution, and expression pattern on monocytes, myeloid cell lines, and AML cells upon culture with different stimuli indicate that B148.4 is a novel monocyte differentiation antigen.
引用
收藏
页码:390 / 398
页数:9
相关论文
共 41 条
[21]   INVITRO DIFFERENTIATION OF HUMAN-MONOCYTES - DIFFERENCES IN MONOCYTE PHENOTYPES INDUCED BY CULTIVATION ON GLASS OR ON COLLAGEN [J].
KAPLAN, G ;
GAUDERNACK, G .
JOURNAL OF EXPERIMENTAL MEDICINE, 1982, 156 (04) :1101-1114
[22]  
KNOWLES DM, 1984, J IMMUNOL, V132, P2170
[23]  
LIU DY, 1986, J IMMUNOL, V137, P448
[24]  
MORGANELLI PM, 1988, J IMMUNOL, V140, P2296
[25]  
NOZAWA R, 1988, BLOOD, V71, P1288
[26]  
PERUSSIA B, 1987, NAT IMMUN CELL GROW, V6, P171
[27]   IMMUNE INTERFERON INDUCES THE RECEPTOR FOR MONOMERIC IGG1 ON HUMAN MONOCYTIC AND MYELOID CELLS [J].
PERUSSIA, B ;
DAYTON, ET ;
LAZARUS, R ;
FANNING, V ;
TRINCHIERI, G .
JOURNAL OF EXPERIMENTAL MEDICINE, 1983, 158 (04) :1092-1113
[28]  
PERUSSIA B, 1983, J IMMUNOL, V130, P2142
[29]  
PERUSSIA B, 1987, J IMMUNOL, V138, P765
[30]  
PERUSSIA B, 1981, BLOOD, V58, P836