NOVEL, HIGHLY DEACTIVATED REVERSED-PHASE FOR BASIC COMPOUNDS

Silica has proven invaluable as a support material for reversed-phase high-performance liquid chromatography. However, contributions of residual acidic silanols lead to poor chromatography of basic compounds. This paper demonstrates the utility of a new, highly deactivated silica-based reversed-phase packing material for the study of a series of weak and strong organic bases. Correlation between retention times on the column and octanol-water partition coefficients at pH 7.0 is demonstrated. Also shown are the results of further experiments in which we investigated the retention characteristics of basic, acidic and neutral compounds by the new deactivated phase. © 1990.