THE EFFECT OF SOME PROTEINASE-INHIBITORS ON LIQUEFACTION OF HUMAN SEMEN

被引：26

作者：

MATSUDA, Y ^{[1
]}

OSHIO, S ^{[1
]}

YAZAKI, T ^{[1
]}

UMEDA, T ^{[1
]}

AKIHAMA, S ^{[1
]}

机构：

[1] TEIKYO UNIV,SCH MED,DEPT UROL,ITABASHI KU,TOKYO,JAPAN

来源：

HUMAN REPRODUCTION | 1994年 / 9卷 / 04期

关键词：

6-AMIDINO-2-NAPHTYL-6-GUANIDINOBENZOATE DIHYDROCHLORIDE; (FUSAN); T-AMINO CAPROIC ACID; HUMAN SEMEN LIQUEFACTION PLASMIN; PROTEINASE INHIBITOR;

D O I：

10.1093/oxfordjournals.humrep.a138568

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

This study examined the effect of some proteinase inhibitors on liquefaction of human semen. It revealed that a strong plasmin inhibitor, 6-amidino-2-naphtyl-6-guanidinobenzoate dihydrochloride (Fusan) showed a significant inhibition of liquefaction, while t-amino caproic acid (t-ACA) showed a weak retardation effect. In terms of sperm quality after liquefaction, Fusan (10 mM), ethyl diamine tetra-acetic acid (EDTA) and Urinastatin completely inhibited sperm motility. Fusan (1 mM) and Lima bean trypsin inhibitor (LBTI) decreased sperm motility significantly, while leupeptin and t-ACA had little effect. Leupeptin, LBTI, t-ACA and Fusan (1 mM) did not affect sperm speed. 50% inhibition of sperm motility was calculated to be similar to 1.7 mM of Fusan concentration. In addition, two inhibitors, Chymostatin and Phosphoramidon were also tested with each experiment and had no effect on liquefaction or on sperm motility and speed. These results strongly suggest that plasmin may play an important role in the liquefaction process of human semen.

引用

页码：664 / 668

页数：5

共 18 条

[1]

ABADI DM, 1969, ENZYMOL BIOL CLIN, V10, P131

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