SIMULTANEOUS SPECTROFLUOROMETRIC DETERMINATION OF GLYCEROL AND ETHANOL IN WINE BY FLOW-INJECTION USING IMMOBILIZED ENZYMES

被引：30

作者：

MATTOS, IL ^{[1
]}

FERNANDEZROMERO, JM ^{[1
]}

DECASTRO, HDL ^{[1
]}

VALCARCEL, M ^{[1
]}

机构：

[1] STATE UNIV CAMPINAS,INST CHEM,CAMPINAS,BRAZIL

来源：

ANALYST | 1995年 / 120卷 / 01期

关键词：

GLYCEROL AND ETHANOL DETERMINATION; IMMOBILIZED ENZYME; FLOW INJECTION; SPECTROFLUOROMETRY; WINE;

D O I：

10.1039/an9952000179

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An automatic spectrofluorimetric method for the simultaneous determination of glycerol and ethanol is presented. The method is based on the use of a flow injection (FI) manifold and dehydrogenase enzyme immobilized on controlled-pore glass. The proposed method allows for the simultaneous determination of glycerol (linear range between 1 and 10 mu g ml(-1), r = 0.9989 and s(r) <1%) and ethanol (linear range between 10 and 100 mu g ml(-1), r = 0.9998 and s(r) <0.9%). It was applied to the determination of both analytes in different wines with recoveries between 97 and 105%. The sampling frequency was 60 samples h(-1). The results obtained on eight wines were compared with those provided by conventional methods and the agreement between them corroborated the usefulness of the proposed method.

引用

页码：179 / 182

页数：4