ISOLATION OF A CDNA CLONE ENCODING A NOVEL MEMBRANE-PROTEIN EXPRESSED IN LYMPHOCYTES

被引：6

作者：

SHIMIZU, Y

OGAWA, H

OKA, Y

MIZUNO, R

SAKODA, S

KISHIMOTO, T

SUGIYAMA, H

机构：

[1] OSAKA UNIV,SCH MED,DEPT MED 3,SUITA,OSAKA 565,JAPAN

[2] OSAKA UNIV,SCH MED,DEPT NEUROL,SUITA,OSAKA 565,JAPAN

来源：

FEBS LETTERS | 1994年 / 355卷 / 01期

关键词：

LYMPHOCYTE-SPECIFIC CDNA; EMBRYONIC STAGE-SPECIFIC CDNA; SUBTRACTIVE HYBRIDIZATION; SINGLE STRANDED PHAGEMID;

D O I：

10.1016/0014-5793(94)01164-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By subtractive hybridization using single-stranded phagemids with directional inserts, we isolated a mouse cDNA clone, LSM-1, from temperature-sensitive Abelson virus-tranformed immature B cells whose differentiation was being induced after the shift from the permissive (35 degrees C) to the non-permissive temperature (39 degrees C). LSM-1, which encodes an as yet unknown peptide of 197 amino acids, has a putative signal sequence and a trans-membrane region, and is expressed in B- and T-cell lines, in spleen, thymus, and bone marrow of adult mice, and in embryos.

引用

页码：30 / 34

页数：5

共 30 条

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[12] ISOLATION AND CHARACTERIZATION OF TEMPERATURE-SENSITIVE MUTANTS OF ABELSON MURINE LEUKEMIA-VIRUS THAT EXHIBIT DISSOCIATION AMONG MORPHOLOGICAL TRANSFORMATION, SOFT AGAR COLONY-FORMING ABILITY AND TYROSINE KINASE-ACTIVITY [J].