A ONE-STEP PROCEDURE FOR PURIFICATION OF BOVINE MAMMARY EPITHELIAL-CELL CD36

被引：8

作者：

GREENWALT, DE

机构：

[1] Holland Laboratory, American Red Cross, Rockville, MD 20855

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1993年 / 4卷 / 01期

关键词：

D O I：

10.1006/prep.1993.1011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple one-step protocol for the purification of the integral membrane protein CD36 from milk-fat-globule membranes of bovine mammary epithelial cells is described. Nonionic detergent extracts of membrane were chromatographed on hydroxylapatite and pure CD36 was eluted with 1 M NaCl. Other proteins of the milk-fat-globule membrane were eluted after CD36 with phosphate buffer. Human platelet CD36 bound to hydroxylapatite only after neuraminidase treatment. CD36 is an extremely hydrophobic and highly glycosylated protein and previous purification procedures have required multiple steps. Chromatography of CD36 on hydroxylapatite provides a simple and quick method of purification which does not sacrifice yield. © 1993 Academic Press, Inc.

引用

页码：72 / 75

页数：4

共 22 条

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