DYSTROPHIN EXPRESSION IN MYOTUBES FORMED BY THE FUSION OF NORMAL AND DYSTROPHIC MYOBLASTS

被引：55

作者：

HUARD, J ^{[1
]}

LABRECQUE, C ^{[1
]}

DANSEREAU, G ^{[1
]}

ROBITAILLE, L ^{[1
]}

TREMBLAY, JP ^{[1
]}

机构：

[1] UNIV LAVAL,HOP ENFANT JESUS,NEUROBIOL LAB,1401 18E RUE,QUEBEC CITY G1J 1Z4,QUEBEC,CANADA

来源：

MUSCLE & NERVE | 1991年 / 14卷 / 02期

关键词：

DYSTROPHIN; MDX MOUSE; MUSCLE CELL CULTURE; HYBRIDIZATION; MUSCULAR DYSTROPHY;

D O I：

10.1002/mus.880140213

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Mdx mouse dystrophy is characterized by the absence in the muscle cytoplasmic membrane of a high molecular weight protein called dystrophin. A possible avenue for treatment of muscular dystrophies is to inject normal myoblasts in a dystrophic muscle to form hybrid muscle fibers. Hybrid myotubes were formed in vitro by the fusion of normal rat and dystrophic mouse (mdx) myoblasts. Staining with Hoechst dye 33258 permitted the clear distinction of mouse and rat nuclei. Immunostaining demonstrated that dystrophin was present over the entire membrane of all hybrid myotubes even when nuclei ratio normal/dystrophic was low.

引用

页码：178 / 182

页数：5

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