SIMULTANEOUS DETERMINATION OF PURINE AND PYRIMIDINE-BASES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION - APPLICATION TO DNA ASSAY

被引：17

作者：

NAKAHARA, T ^{[1
]}

OKUZAWA, M ^{[1
]}

MAEDA, H ^{[1
]}

HIRANO, M ^{[1
]}

MATSUMOTO, T ^{[1
]}

UCHIMURA, H ^{[1
]}

机构：

[1] HIZEN NATL MENTAL HOSP,CTR EMOT & BEHAV DISORDERS,NEUROCHEM LAB,KANZAKI,SAGA 84201,JAPAN

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 10期

关键词：

D O I：

10.1080/10826079208018327

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive method for Simultaneous determination of purine and pyrimidine bases was developed using high performance liquid chromatography with electrochemical detection (ECD). Pyrimidines as well as purines were detectable by ECD under alkaline conditions, and the order of susceptibility to electrochemical oxidation was guanine > adenine > thymine > cytosine. These bases were separated on a vinyl alcohol copolymer gel column and determined by ECD. The detection limit for each base was picomole level. This method was applied to the quantitation of bases in the acid hydrolysates of DNA and oligodeoxy-nucleotides.

引用

页码：1785 / 1796

页数：12

共 11 条

[1] EVALUATION OF COMBINED ELECTROCHEMICAL AND ULTRAVIOLET DETECTION IN THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PLASMA FOR LOW-MOLECULAR-WEIGHT CONSTITUENTS [J].

DWYER, ME ;

BROWN, PR .

JOURNAL OF CHROMATOGRAPHY, 1985, 345 (01) :125-133

[2] BASE AND PHOSPHORUS COMPOSITION ANALYSIS BY GAS CHROMATOGRAPHY [J].

HASHIZUME, T ;

SASAKI, Y .

ANALYTICAL BIOCHEMISTRY, 1968, 24 (02) :232-+

[3]

HURST RO, 1953, J BIOL CHEM, V204, P847

[4] QUANTITATION OF NUCLEIC-ACIDS AT THE PICOGRAM LEVEL USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION [J].

KAFIL, JB ;

CHENG, HY ;

LAST, TA .

ANALYTICAL CHEMISTRY, 1986, 58 (02) :285-289

[5] SPECIFIC AMINO-ACID SUBSTITUTIONS IN BACTERIOOPSIN - REPLACEMENT OF A RESTRICTION FRAGMENT IN THE STRUCTURAL GENE BY SYNTHETIC DNA FRAGMENTS CONTAINING ALTERED CODONS [J].

LO, KM ;

JONES, SS ;

HACKETT, NR ;

KHORANA, HG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (08) :2285-2289

[6]

NAKAHARA T, 1989, ANAL BIOCHEM, V180, P38

[7]

NAKAHARA T, 1990, NEUROCHEM RES, V6, P609

[8] CHROMATOGRAPHIC CHARACTERISTICS OF NUCLEIC-ACID COMPONENTS ON VINYL ALCOHOL COPOLYMER GEL COLUMNS [J].

NOGUCHI, K ;

YANAGIHARA, Y ;

KASAI, M ;

HIRATA, N .

JOURNAL OF CHROMATOGRAPHY, 1990, 506 :145-155

[9]

PULLMAN B, 1963, QUANTUM BIOCHEMISTRY, P215

[10] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC PROFILING OF NUCLEIC-ACID COMPONENTS IN PHYSIOLOGICAL SAMPLES [J].

SIMPSON, RC ;

BROWN, PR .

JOURNAL OF CHROMATOGRAPHY, 1986, 379 :269-311

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