SIMULTANEOUS DETERMINATION OF PURINE AND PYRIMIDINE-BASES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION - APPLICATION TO DNA ASSAY

被引：17

作者：

NAKAHARA, T ^{[1
]}

OKUZAWA, M ^{[1
]}

MAEDA, H ^{[1
]}

HIRANO, M ^{[1
]}

MATSUMOTO, T ^{[1
]}

UCHIMURA, H ^{[1
]}

机构：

[1] HIZEN NATL MENTAL HOSP,CTR EMOT & BEHAV DISORDERS,NEUROCHEM LAB,KANZAKI,SAGA 84201,JAPAN

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 10期

关键词：

D O I：

10.1080/10826079208018327

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive method for Simultaneous determination of purine and pyrimidine bases was developed using high performance liquid chromatography with electrochemical detection (ECD). Pyrimidines as well as purines were detectable by ECD under alkaline conditions, and the order of susceptibility to electrochemical oxidation was guanine > adenine > thymine > cytosine. These bases were separated on a vinyl alcohol copolymer gel column and determined by ECD. The detection limit for each base was picomole level. This method was applied to the quantitation of bases in the acid hydrolysates of DNA and oligodeoxy-nucleotides.

引用

页码：1785 / 1796

页数：12