PURIFICATION AND CHARACTERIZATION OF IDURONIC ACID-RICH AND GLUCURONIC ACID-RICH PROTEOGLYCANS IMPLICATED IN HUMAN POSTBURN KELOID SCAR

Small proteoglycans (PGs), extracted from human keloid scar tissue with 4m guanidinium chloride and fractionated by DEAE-cellulose chromatography, were separated by ethanol precipitation into one l-iduronic acid-rich and one d-glucuronic acid-rich fraction. The size of the l-iduronic acid-rich PG was 102 kDa with a 27 kDa glycosaminoglycan chain, that of the d-glucuronic acid-rich PG was 90 kDa with a 26 kDa glycosaminoglycan chain, and the protein core of both PGs was 14.5 kDa. The two PGs carried sulfate groups mostly attached at C-4 of the 2-amino-2-deoxy-d-galactose units. The n-terminal amino acid sequence of both was similar to human bone PGII (decorin), normal and hypertrophic scar, and human dermal tissue PG. © 1990.