CLONING, NUCLEOTIDE-SEQUENCE, AND TRANSCRIPTIONAL ANALYSIS OF THE PEDIOCOCCUS-ACIDILACTICI L-(+)-LACTATE DEHYDROGENASE GENE

被引：36

作者：

GARMYN, D ^{[1
]}

FERAIN, T ^{[1
]}

BERNARD, N ^{[1
]}

HOLS, P ^{[1
]}

DELCOUR, J ^{[1
]}

机构：

[1] UNIV CATHOLIQUE LOUVAIN, GENET MOLEC LAB, UNITE GENET, B-1348 LOUVAIN, BELGIUM

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 01期

关键词：

D O I：

10.1128/AEM.61.1.266-272.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant plasmids containing the Pediococcus acidilactici L-(+)-lactate dehydrogenase gene (ldhL) were isolated by complementing for growth under anaerobiosis of an Escherichia coli lactate dehydrogenase-pyruvate formate lyase double mutant. The nucleotide sequence of the ldhL gene predicted a protein of 323 amino acids showing significant similarity with other bacterial L-(+)-lactate dehydrogenases and especially with that of Lactobacillus plantarum. The ldhL transcription start points in P. acidilactici were defined by primer extension, and the promoter sequence was identified as TCAACT-(17 bp)-TATAAT. This sequence is closely related to the consensus sequence of vegetative promoters from gram-positive bacteria as well as from E. coli. Northern analysis of P. acidilactici RNA showed a 1.1-kb ldhL transcript whose abundance is growth rate regulated. These data, together with the presence of a putative rho-independent transcriptional terminator, suggest that ldhL is expressed as a monocistronic transcript in P. acidilactici.

引用

页码：266 / 272

页数：7

共 62 条

[11] FROM ANALYSIS TO SYNTHESIS - NEW LIGAND-BINDING SITES ON THE LACTATE-DEHYDROGENASE FRAMEWORK .1. [J].

CLARKE, AR ;

ATKINSON, T ;

HOLBROOK, JJ .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (03) :101-105

[12] A SINGLE AMINO-ACID SUBSTITUTION DEREGULATES A BACTERIAL LACTATE-DEHYDROGENASE AND STABILIZES ITS TETRAMERIC STRUCTURE [J].

CLARKE, AR ;

WIGLEY, DB ;

BARSTOW, DA ;

CHIA, WN ;

ATKINSON, T ;

HOLBROOK, JJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 913 (01) :72-80

[13] THE ASSEMBLY MECHANISM OF THE LACTATE-DEHYDROGENASE TETRAMER FROM BACILLUS-STEAROTHERMOPHILUS - THE EQUILIBRIUM RELATIONSHIPS BETWEEN QUATERNARY STRUCTURE AND THE BINDING OF FRUCTOSE-1,6-BISPHOSPHATE, NADH AND OXAMATE [J].

CLARKE, AR ;

ATKINSON, T ;

CAMPBELL, JW ;

HOLBROOK, JJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 829 (03) :387-396

[14] THE MOLECULAR PATHWAY BY WHICH FRUCTOSE-1,6-BISPHOSPHATE INDUCES THE ASSEMBLY OF A BACTERIAL LACTATE-DEHYDROGENASE [J].

CLARKE, AR ;

EVINGTON, JRN ;

DUNN, CR ;

ATKINSON, T ;

HOLBROOK, JJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 870 (01) :112-126

[15]

COLLINS MD, 1991, FEMS MICROBIOL LETT, V77, P5, DOI [10.1016/S0378-1097(99)00289-X, 10.1111/j.1574-6968.1991.tb04313.x]

[16] THE PHYLOGENY OF AEROCOCCUS AND PEDIOCOCCUS AS DETERMINED BY 16S RIBOSOMAL-RNA SEQUENCE-ANALYSIS - DESCRIPTION OF TETRAGENOCOCCUS GEN-NOV [J].

COLLINS, MD ;

WILLIAMS, AM ;

WALLBANKS, S .

FEMS MICROBIOLOGY LETTERS, 1990, 70 (03) :255-262

[17] CLONING OF A LACTATE-DEHYDROGENASE GENE FROM CLOSTRIDIUM-ACETOBUTYLICUM-B643 AND EXPRESSION IN ESCHERICHIA-COLI [J].

CONTAG, PR ;

WILLIAMS, MG ;

ROGERS, P .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (12) :3760-3765

[18] FRUCTOSE 1,6-DIPHOSPHATE-ACTIVATED L-LACTATE DEHYDROGENASE FROM STREPTOCOCCUS-LACTIS - KINETIC-PROPERTIES AND FACTORS AFFECTING ACTIVATION [J].

CROW, VL ;

PRITCHARD, GG .

JOURNAL OF BACTERIOLOGY, 1977, 131 (01) :82-91

[19] DNA-SEQUENCE AND INVITRO MUTAGENESIS OF THE GENE ENCODING THE FRUCTOSE-1,6-DIPHOSPHATE-DEPENDENT L-(+)-LACTATE DEHYDROGENASE OF STREPTOCOCCUS-MUTANS [J].

DUNCAN, MJ ;

HILLMAN, JD .

INFECTION AND IMMUNITY, 1991, 59 (11) :3930-3934

[20] THE OMPA 5' UNTRANSLATED RNA SEGMENT FUNCTIONS IN ESCHERICHIA-COLI AS A GROWTH-RATE-REGULATED MESSENGER-RNA STABILIZER WHOSE ACTIVITY IS UNRELATED TO TRANSLATIONAL EFFICIENCY [J].

EMORY, SA ;

BELASCO, JG .

JOURNAL OF BACTERIOLOGY, 1990, 172 (08) :4472-4481

← 1 2 3 4 5 6 7 →