NONENZYMATIC VERSUS ENZYMATIC HEPATOCYTE ISOLATION FROM PIG LIVERS FOR LARGER SCALE INVESTIGATIONS OF LIVER-CELL PERFUSION SYSTEMS

被引：17

作者：

GERLACH, JC ^{[1
]}

BROMBACHER, J ^{[1
]}

COURTNEY, JM ^{[1
]}

NEUHAUS, P ^{[1
]}

机构：

[1] UNIV STRATHCLYDE,BIOENGN UNIT,GLASGOW,LANARK,SCOTLAND

来源：

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS | 1993年 / 16卷 / 09期

关键词：

HEPATOCYTE ISOLATION; COLLAGENASE; EDTA; BIOREACTORS; CELL PERFUSION SYSTEMS; HYBRID LIVER SUPPORT; ARTIFICIAL LIVER;

D O I：

10.1177/039139889301600909

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A comparison of nonenzymatic and enzymatic hepatocyte isolation was performed on pig livers. The collagenase perfusion showed superior results: mean viability 72 +/- 10% versus a maximum viability of 21% using EDTA-perfusion. A five-step collagenase perfusion technique, developed for pig livers enables larger scale investigations, in order to develop methods for hepatocyte cultures in therapeutical liver cell perfusion systems.

引用

页码：677 / 681

页数：5

共 25 条

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