NONENZYMATIC VERSUS ENZYMATIC HEPATOCYTE ISOLATION FROM PIG LIVERS FOR LARGER SCALE INVESTIGATIONS OF LIVER-CELL PERFUSION SYSTEMS

被引：17

作者：

GERLACH, JC ^{[1
]}

BROMBACHER, J ^{[1
]}

COURTNEY, JM ^{[1
]}

NEUHAUS, P ^{[1
]}

机构：

[1] UNIV STRATHCLYDE,BIOENGN UNIT,GLASGOW,LANARK,SCOTLAND

来源：

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS | 1993年 / 16卷 / 09期

关键词：

HEPATOCYTE ISOLATION; COLLAGENASE; EDTA; BIOREACTORS; CELL PERFUSION SYSTEMS; HYBRID LIVER SUPPORT; ARTIFICIAL LIVER;

D O I：

10.1177/039139889301600909

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A comparison of nonenzymatic and enzymatic hepatocyte isolation was performed on pig livers. The collagenase perfusion showed superior results: mean viability 72 +/- 10% versus a maximum viability of 21% using EDTA-perfusion. A five-step collagenase perfusion technique, developed for pig livers enables larger scale investigations, in order to develop methods for hepatocyte cultures in therapeutical liver cell perfusion systems.

引用

页码：677 / 681

页数：5

共 25 条

[11] ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER
HOWARD, RB
CHRISTENSEN, AK
GIBBS, FA
PESCH, LA
[J]. JOURNAL OF CELL BIOLOGY, 1967, 35 (03) : 675 - +
[12] JAUREGUI HO, 1988, IN VITRO CELL DEV, V5, P24
[13] Kissmeyer-Nielsen F., 1967, LYMPHOCYTOTOXIC MICR, P381
[14] LENZI M, 1985, LIVER, V5, P212
[15] LONGMUIR A, 1956, NATURE, V177, P997
[16] RAT HEPATOCYTES PREPARED WITHOUT COLLAGENASE - PROLONGED RETENTION OF DIFFERENTIATED CHARACTERISTICS IN CULTURE
MEREDITH, MJ
[J]. CELL BIOLOGY AND TOXICOLOGY, 1988, 4 (04) : 405 - 425
[17] MULLER M, 1972, CANCER RES, V32, P2568
[18] THE NATURE OF THE GOLGI APPARATUS .1. PARALLELISM BETWEEN INTERCELLULAR MYELIN FIGURES AND GOLGI APPARATUS IN SOMATIC CELLS
PALADE, GE
CLAUDE, A
[J]. JOURNAL OF MORPHOLOGY, 1949, 85 (01) : 35 - 69
[19] VIABILITY OF CELLS GROWN OR CENTRIFUGED IN A NEW DENSITY GRADIENT MEDIUM, PERCOLL(TM)
PERTOFT, H
RUBIN, K
KJELLEN, L
LAURENT, TC
KLINGEBORN, B
[J]. EXPERIMENTAL CELL RESEARCH, 1977, 110 (02) : 449 - 457
[20] EFFECT OF DIFFERENT COLLAGENASES ON THE ISOLATION OF VIABLE HEPATOCYTES FROM RAT-LIVER
QUERAL, AE
DEANGELO, AB
GARRETT, CT
[J]. ANALYTICAL BIOCHEMISTRY, 1984, 138 (01) : 235 - 237

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