MODEL OF TRANSLATIONAL CONTROL INVOLVING MESSENGER RNA-ASSOCIATED PROTEINS IN CHICK EMBRYONIC MUSCLES

被引：20

作者：

JAIN, SK

ROY, RK

PLUSKAL, MG

CROALL, DE

GUHA, C

SARKAR, S

机构：

[1] BOSTON BIOMED RES INST, DEPT MUSCLE RES, 20 STANIFORD ST, BOSTON, MA 02114 USA

[2] HARVARD UNIV, SCH MED, DEPT NEUROL, BOSTON, MA 02115 USA

来源：

MOLECULAR BIOLOGY REPORTS | 1979年 / 5卷 / 1-2期

关键词：

D O I：

10.1007/BF00777492

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Highly purified poly(A)-containing free and polysomal mRNP particles have been isolated by chromatography of subcellular fractions of chick embryonic muscles on oligo-dT-cellulose and elution with low salt buffer at 45°. The free and polysomal mRNP represent two distinct classes of macromolecules, the free particles having a more complex nucleoprotein organization than the polysomal particles. Comparison of the protein moieties of three classes of poly(A)-containing cytoplasmic mRNP - those released from nuclei after in vitro transcription and processing (transported mRNP), the free, and polysomal mRNP - strongly suggests that the majority of the mRNA-associated proteins are exchanged in the cytoplasm during the various functional states of mRNA. A model of translational control involving the participation of mRNA-associated proteins in chick embryonic muscles and by analogy in other differentiated eukaryotic cells is proposed. © 1979 Dr. W. Junk bv Publishers.

引用

页码：79 / 85

页数：7

共 21 条

[11] THERMAL CHROMATOGRAPHY OF EUKARYOTIC MESSENGER-RIBONUCLEOPROTEIN PARTICLES ON OLIGO(DT)-CELLULOSE - EVIDENCE FOR COMMON MESSENGER RNA-ASSOCIATED PROTEINS IN VARIOUS CELL-TYPES [J].