EPITOPE MAPPING OF APAMIN BY MEANS OF MONOCLONAL-ANTIBODIES RAISED AGAINST FREE OR CARRIER-COUPLED PEPTIDE

被引：5

作者：

DEFENDINI, ML ^{[1
]}

PIERRES, M ^{[1
]}

REGNIERVIGOUROUX, A ^{[1
]}

ROCHAT, H ^{[1
]}

GRANIER, C ^{[1
]}

机构：

[1] CNRS,INSERM,CTR IMMUNOL,F-13288 MARSEILLE,FRANCE

来源：

MOLECULAR IMMUNOLOGY | 1990年 / 27卷 / 06期

关键词：

D O I：

10.1016/0161-5890(90)90074-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A panel of 20 monoclonal antibodies raised against the bee-venom peptide apamin (18 residues, 2 disulfide bridges) was prepared. Nine monoclonal antibodies (mAb) were obtained from a mouse immunized with free apamin and 11 from a mouse immunized with a mixture of free and carrier-coupled peptide. Using a panel of 11 synthetic apamin analogs, we examined the fine antigenic specificity of each antibody. The mAb generated against free apamin preferentially bound to the central part of the peptide and less frequently recognized the N- and C-terminal regions. However, monoclonal antibodies obtained by immunization with carrier-bound apamin showed a broader range of specificities, consistent with the possibility of the entire surface of this small antigen becoming immunogenic upon coupling to the carrier. © 1990.

引用

页码：551 / 558

页数：8

共 20 条

[11] CONSTRAINTS IN T-B COOPERATION RELATED TO EPITOPE TOPOLOGY ON ESCHERICHIA-COLI BETA-GALACTOSIDASE .1. THE FINE SPECIFICITY OF T-CELLS DICTATES THE FINE SPECIFICITY OF ANTIBODIES DIRECTED TO CONFORMATION-DEPENDENT DETERMINANTS [J].