IMPROVEMENT OF S-ADENOSYLMETHIONINE PRODUCTION BY INTEGRATION OF THE ETHIONINE-RESISTANCE GENE INTO CHROMOSOMES OF THE YEAST SACCHAROMYCES-CEREVISIAE

被引：36

作者：

SHIOMI, N

FUKUDA, H

MURATA, K

KIMURA, A

机构：

[1] KYOTO UNIV,FOOD SCI RES INST,DEPT APPL MICROBIOL,UJI,KYOTO 611,JAPAN

[2] KANEKA CORP,ENGN RES LABS,TAKASAGO,HYOGO 676,JAPAN

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1995年 / 42卷 / 05期

关键词：

D O I：

10.1007/BF00171953

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An ethionine-resistance gene cloned from Saccharomyces cerevisiae DKD-5D-H was able to enhance S-adenosyl-L-methionine (AdoMet) accumulation when it was introduced into the yeast cells on multi-copy plasmid YEp13. In order to increase the AdoMet accumulation, the gene was integrated into the yeast chromosome by using a yeast transposon Ty element. When the YEp plasmid was used for the integration, the ethionine-resistance gene was efficiently inserted into the yeast chromosomes with a substantial increase in AdoMet productivity (about twofold) in comparison with that by the yeast cells carrying the gene on an extrachromosomal multi-copy plasmid.

引用

页码：730 / 733

页数：4

共 11 条

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