CHARACTERIZATION OF A 3RD MAJOR SINE FAMILY OF REPETITIVE SEQUENCES IN THE GALAGO GENOME

被引:38
作者
DANIELS, GR
DEININGER, PL
机构
[1] ALTON OCHSNER MED FDN & OCHSNER CLIN,MOLEC GENET LAB,NEW ORLEANS,LA 70121
[2] LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,NEW ORLEANS,LA 70112
关键词
D O I
10.1093/nar/19.7.1649
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In addition to the Alu family of short interspersed repetitive DNA elements (SINEs), we have previously characterized one other repetitive DNA family (Type II) in the prosimian, Galago crassicaudatus. We present here a detailed analysis of seventeen members of a third galago SINE family designated as the Monomer family. Both the Monomer and Type II families are shown to be specific for the galago genome as compared to other primates, including another prosimian, the lemur. Moreover, in vitro transcription of galago SINEs suggests that the Monomer and Type II families have appreciably stronger RNA polymerase III promoters than does the Alu family. This agrees with the promoter sequence for each of these SINE families, in that the Monomer and Type II family promoters are more closely related to the RNA polymerase III promoter consensus sequence than is the Alu family promoter. These promoter strength analyses also correlate with copy number and sequence divergence analyses, which suggests that the SINE families with the strongest promoters have been amplified most recently in the galago genome.
引用
收藏
页码:1649 / 1656
页数:8
相关论文
共 36 条
[31]   CLONING IN SINGLE-STRANDED BACTERIOPHAGE AS AN AID TO RAPID DNA SEQUENCING [J].
SANGER, F ;
COULSON, AR ;
BARRELL, BG ;
SMITH, AJH ;
ROE, BA .
JOURNAL OF MOLECULAR BIOLOGY, 1980, 143 (02) :161-178
[32]  
SLAGEL V, 1987, MOL BIOL EVOL, V4, P19
[33]  
TRAINA V, 1983, BRL FOCUS, V5, P5
[34]   ALU SEQUENCES ARE PROCESSED 7SL RNA GENES [J].
ULLU, E ;
TSCHUDI, C .
NATURE, 1984, 312 (5990) :171-172
[35]   DIRECT REPEATS FLANK 3 SMALL NUCLEAR-RNA PSEUDOGENES IN THE HUMAN GENOME [J].
VANARSDELL, SW ;
DENISON, RA ;
BERNSTEIN, LB ;
WEINER, AM ;
MANSER, T ;
GESTELAND, RF .
CELL, 1981, 26 (01) :11-17
[36]   NONVIRAL RETROPOSONS - GENES, PSEUDOGENES, AND TRANSPOSABLE ELEMENTS GENERATED BY THE REVERSE FLOW OF GENETIC INFORMATION [J].
WEINER, AM ;
DEININGER, PL ;
EFSTRATIADIS, A .
ANNUAL REVIEW OF BIOCHEMISTRY, 1986, 55 :631-661