PROPERTIES OF THE NA+/H+ ANTIPORTER IN VIBRIO-PARAHAEMOLYTICUS

被引：21

作者：

KURODA, T ^{[1
]}

SHIMAMOTO, T ^{[1
]}

INABA, K ^{[1
]}

KAYAHARA, T ^{[1
]}

TSUDA, M ^{[1
]}

TSUCHIYA, T ^{[1
]}

机构：

[1] OKAYAMA UNIV,FAC PHARMACEUT SCI,DEPT MICROBIOL,OKAYAMA 700,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1994年 / 115卷 / 06期

关键词：

NA+/H+ ANTIPORTER; PH-DEPENDENCE; PROPERTIES; SUBSTRATE SPECIFICITY; VIBRIO PARAHAEMOLYTICUS;

D O I：

10.1093/oxfordjournals.jbchem.a124473

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The properties of the Na+/H+ antiporter in Vibrio parahaemolyticus, a slightly halophilic bacterium, were investigated using everted membrane vesicles. It appears that at least two Na+/H+ antiporters are present, one that is pH-dependent and one that is pH-independent. These two antiporters appear to correspond to the NhaA and NhaB antiporters of Escherichia coli, respectively. It seems that amiloride strongly inhibits the pH-dependent antiporter. Na+ is the best substrate for both of the two V. parahaemolyticus antiporters. Li+ is a poorer substrate and K+ is not a substrate. No K+/H+ antiport activity was detected in membrane vesicles of this organism. The Na+(Li+)/H+ antiport activity greatly increased with an increase in pH of the assay medium. pH did not affect the K-m value of the Na+/H+ antiport, but it did increase the V-max.

引用

页码：1162 / 1165

页数：4