FLUORESCENCE INSITU HYBRIDIZATION WITH HUMAN CHROMOSOME-SPECIFIC LIBRARIES - DETECTION OF TRISOMY-21 AND TRANSLOCATIONS OF CHROMOSOME-4

被引：1242

作者：

PINKEL, D

LANDEGENT, J

COLLINS, C

FUSCOE, J

SEGRAVES, R

LUCAS, J

GRAY, J

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1988年 / 85卷 / 23期

关键词：

D O I：

10.1073/pnas.85.23.9138

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chromosomes can be specifically stained in metaphase spreads and interphase nuclei by in situ hybridization with entire chromosome-specific DNA libraries. Unlabeled human genomic DNA is used to inhibit the hybridization of sequences in the library that bind to multiple chromosomes. The target chromosome can be made at least 20 times brighter per unit length than the others. Trisomy 21 and translocations involving chromosome 4 can be detected in metaphase spreads and interphase nuclei by using this technique.

引用

页码：9138 / 9142

页数：5

共 24 条

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