MOLECULAR GENETIC-ANALYSIS REVEALS A PUTATIVE BIFUNCTIONAL POLYKETIDE CYCLASE DEHYDRASE GENE FROM STREPTOMYCES-COELICOLOR AND STREPTOMYCES-VIOLACEORUBER, AND A CYCLASE O-METHYLTRANSFERASE FROM STREPTOMYCES-GLAUCESCENS

被引：37

作者：

SHERMAN, DH

BIBB, MJ

SIMPSON, TJ

JOHNSON, D

MALPARTIDA, F

FERNANDEZMORENO, M

MARTINEZ, E

HUTCHINSON, CR

HOPWOOD, DA

机构：

[1] UNIV MINNESOTA,INST ADV STUDIES BIOL PROC TECHNOL,ST PAUL,MN 55108

[2] UNIV MINNESOTA,DEPT MICROBIOL,ST PAUL,MN 55108

[3] UNIV BRISTOL,DEPT CHEM,BRISTOL BS8 1TS,AVON,ENGLAND

[4] UNIV MINNESOTA,CTR MOLEC BIOL COMP,ST PAUL,MN 55108

[5] CTR NACL BIOTECN,E-28006 MADRID,SPAIN

[6] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706

[7] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706

来源：

TETRAHEDRON | 1991年 / 47卷 / 31期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0040-4020(01)86494-2

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Molecular genetic analysis of the actinorhodin gene cluster from Streptomyces coelicolor has revealed a putative bifunctional cyclase/dehydrase gene. Open reading frame (ORF) 4 from both the actinorhodin (act) and granaticin (gra) polyketide synthase (PKS) gene clusters were able to relieve the block in a mutant strain of S. coelicolor which produces mutactin. This compound is a shunt product of the actinorhodin biosynthetic pathway which results from an aberrant intramolecular aldol cyclization, and a failure to dehydrate the hydroxyl group at C-9. These results provide compelling evidence for the existence of a novel polyketide aldolase which specifies the correct cyclization of a complex oligoketide chain.

引用

页码：6029 / 6043

页数：15

共 57 条

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