DIETARY VITAMIN-E DECREASES ESR SIGNAL INTENSITY IN HEPATIC-MICROSOMAL PREPARATIONS FROM MALIGNANT HYPERTHERMIA SUSCEPTIBLE PIGS

On incubation with the spin trap α4-pyridyl-l-oxide)-N-tert-butylnitrone (4-POBN), a characteristic electron spin resonance (ESR) signal was produced at a greater rate in hepatic microsomal fractions from malignant hyprthermia susceptible (MHS) pigs compared with resistant (MHR) pigs. This was accompanied by increased formation of thiobarbituric acid reactive substances (TBARS). Supplementation of diets for six weeks with 235 mg αtocopherol acetate/kg significantly increased microsomal vitamin E content of both pig types. Moreover, the rate of formation of TBARS and ESR signal height of incubated microsomes from supplemented MHS pigs was decreased to that of MHR pigs. Elevated pyruvate kinase activities and TBARS concentrations in plasma of MHS pigs were also moderated by dietary vitamin E. Vitamin E supplementation may decrease the peroxidative events associated with MH. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.