CYTOCHEMISTRY OF HUMAN CATALASE - DEMONSTRATION OF HEPATIC AND RENAL PEROXISOMES BY A HIGH-TEMPERATURE PROCEDURE

被引:93
作者
ROELS, F
GOLDFISCHER, S
机构
[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PATHOL,BRONX,NY 10461
[2] YESHIVA UNIV ALBERT EINSTEIN COLL MED,LIVER RES CTR,BRONX,NY 10461
[3] STATE UNIV GHENT,MENSELIJKE ANAT LAB,B-9000 GHENT,BELGIUM
关键词
D O I
10.1177/27.11.92501
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The cytochemical demonstration of marker enzymes for subcellular organelles permits light microscopic analysis of their structure and function in normal and diseased tissues. Currently available staining procedures for the peroxidase activity of catalase in peroxisomes of plant and animal cells yield weak and inconsistent light microscopic staining when applied to human tissues. We have developed a simple and sensitive high temperature procedure that clearly and reproducibly stains these abundant, but poor understood, organelles in biopsy specimens of human liver and kidney. This method utilizes formaldehyde fixation, a modified diaminobenzine (DAB) medium, incubation at 45°C and postosmication for both light and electron microscopy.
引用
收藏
页码:1471 / 1477
页数:7
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